FastQCFastQC Report
Tue 18 Jun 2019
HHJHYBGXB_n01_Guide.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHJHYBGXB_n01_Guide.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15997335
Sequences flagged as poor quality0
Sequence length28
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAAGA122150.020.6342054
AAGAGCA119800.020.2355257
GAAGAGC120350.019.9555536
AGTGAAG133000.019.6791213
TGAAGAG122350.019.3685825
AGAGCAA128100.019.2676168
AGCAACC126650.018.9240110
CGAAAAG47750.018.9161473
AAAGTGA153350.018.8354641
ACGAAAC65150.018.4742072
GAGCAAC130600.018.3684379
AAACGAA101400.018.1221311
CGCGCCA28800.017.9500269
ACGAAAA45050.017.3390962
GAGAAAT127550.017.322641
ACGAACA44650.017.297313
AAGTGAA149950.017.0731162
AACGAAA154400.017.0468711
CTGGACG25800.016.974391
AGAACCA53250.016.9210453
Produced by FastQC (version 0.11.5)