FastQCFastQC Report
Thu 13 Jun 2019
HHJHWBGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHJHWBGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20389504
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG602570.29552950380744913No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG72900.013.67787551
CGGGGGG68250.010.9058891
TGGGGGG54000.09.5576561
CGGTAGT23150.06.34951230
TCCGGTC28600.06.24051810
CTAGCGT27500.06.23599129
CCGGTAG24300.06.193045629
AGTTAGG32250.06.07676911
GCACACG53400.06.03051970
TAACGCG16304.947651E-106.011807457
CGGCCTA17507.4578566E-115.999229417
GCGTTCC24000.05.98033436
CCGGTCC29400.05.951645411
ATCGGCG29600.05.9144052
GTTCCGG26800.05.87693268
GATCGGC29950.05.86139541
CCGTAGT29350.05.72411357
CGTAGTT36400.05.6731418
TGTGCGG22301.8189894E-125.6513286
CCCGTAA20602.7284841E-115.60618638
Produced by FastQC (version 0.11.5)