FastQCFastQC Report
Thu 13 Jun 2019
HHJHWBGXB_n01_KN1-StrepInput.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHJHWBGXB_n01_KN1-StrepInput.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32089408
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG53950.011.9396042
GGCGTTC52750.011.87951855
GCGTTCC55750.011.42876
GATCGGC58700.011.0939041
CGTATGC20950.010.85885246
GTTCCGG62100.010.14592558
TCCGGTC62500.010.08001710
CCGGTCC64750.09.67549611
CGGTCCA68450.09.20355612
CGGTAGT72750.08.75570230
CCGTAGT142200.08.6155297
GCCGTAG144250.08.5184716
GTAGCCG146700.08.4713043
TAGGGCC73450.08.43398323
GTTAGGC148000.08.39504912
GTTAGGG78450.08.20870721
TAACGCG60150.08.14603657
AGTTAGG155800.08.1320711
TGTAGCC157200.08.1283772
TAGGCCA156850.08.0551814
Produced by FastQC (version 0.11.5)