FastQCFastQC Report
Thu 13 Jun 2019
HHJHWBGXB_n01_KN1-GRChip.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHJHWBGXB_n01_KN1-GRChip.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28255473
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTCC77300.09.7370946
CGGTAGT89000.08.92668430
GGCGTTC85550.08.9208485
GATCGGC90800.08.7532091
TAGGGCC88850.08.74469223
ATCGGCG88800.08.7125312
TTCCGGT96400.08.1325669
TTAGGGC95750.08.11452622
TCCGGTC97050.07.933832610
GGTCCAT99700.07.79303813
GGTTAGG100850.07.773566720
GTTCCGG99250.07.65290988
ATACGCT102200.07.63673225
TTAAACG116350.07.58032326
GTTAGGG100400.07.564395421
TAAACGC117100.07.561661227
TGTATAC164850.07.536904325
TCGAGTA118800.07.4848234
CCGGTCC105800.07.47610811
TACTGTA71950.07.393803643
Produced by FastQC (version 0.11.5)