FastQCFastQC Report
Thu 13 Jun 2019
HHJHWBGXB_n01_GIF1-StrepInput.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHJHWBGXB_n01_GIF1-StrepInput.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26464587
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC98600.07.10006670
CACACGT110750.06.60555769
GCGTTCC52400.06.48016266
CCGTAGT96350.06.3210687
ATCGGCG51900.06.27284152
AACGCGG93900.06.149919515
GTTAGGC98650.06.031212
GATCGGC57050.06.0142171
GTAGCCG102750.05.9960893
CGTCTGA79400.05.99553270
GCCGTAG101550.05.96364266
GCACACG121850.05.94632268
GTTCCGG58950.05.8187698
CCGCCTA102450.05.73924843
TAGGCCA105900.05.71742414
CGCTGTA88250.05.71206576
TTAGGCC108750.05.696318613
ACGCTGT88550.05.69269235
AGTTAGG110000.05.631619511
TCCGGTC60200.05.523102310