FastQCFastQC Report
Thu 13 Jun 2019
HHJHWBGXB_n01_GFPStrep-StrepInput.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHJHWBGXB_n01_GFPStrep-StrepInput.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32939590
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC24050.09.31362746
GGCGTTC62650.06.42606075
GCACACG134350.06.35702170
GCGTTCC62450.06.33456376
ACACGTC108500.06.258546469
CGTCTGA92200.05.88440170
CACACGT123300.05.649258670
GTATGCC42850.05.635847
GATCGGC70750.05.59185171
CACACTG41000.05.46320531
TCCGGTC74300.05.322947510
CCGGTCC75050.05.26970611
GTTCCGG73250.05.1609488
TCTGAAC93050.05.11592370
TCGGAAG287650.05.098601370
CCGTCTT43700.05.045641452
CGGTCCA81600.05.018242412