FastQCFastQC Report
Thu 13 Jun 2019
HHJHWBGXB_n01_GFPGR-GRInput.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHJHWBGXB_n01_GFPGR-GRInput.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26424190
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTCC78400.09.5555186
GGCGTTC79200.09.4147255
GATCGGC81750.09.207831
TCCGGTC85150.08.7549510
CCGGTCC89400.08.49532711
GTTCCGG87600.08.470818
TTCCGGT91400.08.0414649
CGGTCCA98900.07.997760312
CCGGTAG94650.07.691256529
CCGTAGT74850.07.66920237
GGTCCAT99700.07.65269313
CGGTAGT98150.07.630954730
TAGGGCC96000.07.619541623
GTTAGGC77250.07.566137312
CCCGTAA73950.07.477780338
TAACGCG46100.07.4401357
GTTAGGG102600.07.36816521
CGTAATG75850.07.290479740
TTAGGGC102050.07.23641122
ACGCTAT117900.07.15418727