FastQCFastQC Report
Thu 13 Jun 2019
HHJHWBGXB_n01_GFPGR-GRChip.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHJHWBGXB_n01_GFPGR-GRChip.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25405025
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCATAG102650.09.8878899
GCGTTCC68900.09.8573436
CCGGTAG75250.09.394958529
GGCGTTC73800.09.345065
CCATAGA111200.09.03321810
CGGTAGT78200.08.95105330
TTCCGGT81250.08.9168519
TACTGTA68600.08.87732243
TGTATAC142400.08.82338725
GTTCCGG81900.08.804378
GTTAGCC109800.08.79852270
TAGGGCC78900.08.78292223
GATCGGC82650.08.7688291
TTAGGGC83150.08.5444622
TCCGGTC82150.08.39313110
TAGATCA121650.08.31452513
GTTAGGG86500.08.213521
GGTCCAT89750.08.11111513
AATCCGT84200.08.06387759
TCATACG102200.08.047626523
Produced by FastQC (version 0.11.5)