FastQCFastQC Report
Thu 13 Jun 2019
HHJHWBGXB_n01_5958-StrepInput.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHJHWBGXB_n01_5958-StrepInput.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35234923
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC32300.011.70240544
GGCGTTC53250.08.4805825
GCGTTCC52350.08.2251886
AACGCGG141650.07.634701715
ATCGGCG57050.07.6088092
TTACGGT142200.07.55604427
GTTAGGC145850.07.486900312
CGGTAGT60150.07.33145230
CCGGTAG56850.07.326068429
ACGCTGT131950.07.26936965
ACGCGGC147350.07.268109316
TAACGCG45600.07.214728457
CGCTGTA134100.07.1267466
TCCGGTC62550.07.050249610
ACGGTTC153100.06.972349629
AGTTAGG158600.06.929244511
CCGTCTT58150.06.921508350
TAGACTG167400.06.899318720
TAGGCCA158700.06.880624314
TTAGGCC158750.06.878486613
Produced by FastQC (version 0.11.5)