FastQCFastQC Report
Thu 13 Jun 2019
HHJHWBGXB_n01_5958-GRChip.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHJHWBGXB_n01_5958-GRChip.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23628968
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAGTA98200.08.8051154
GCGTTCC64400.08.4256376
GTTTCGA104750.08.3557271
TTAAACG99100.08.26414726
TAAACGC100950.08.14736927
CGGTAGT69550.08.00121330
CTGTTAA104100.07.93435523
CCGGTAG67650.07.9155229
TTCGAGT107500.07.88043263
CGAGTAG128400.07.79752975
TACTGTA57050.07.791158743
GGCGTTC71650.07.76849655
TAATGCC110900.07.668823213
AGTAGAT111800.07.57676127
GATCGGC80150.07.5568521
TAGGGCC74100.07.462583523
ATCGGCG79900.07.4482382
TCCGGTC78200.07.384828610
GTAGATA118050.07.38303958
TACGCTA81550.07.38176726