FastQCFastQC Report
Mon 24 Sep 2018
HHJCJBGX7_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHJCJBGX7_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28308661
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG824990.29142671212884286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC604150.054.9764213
CGTCTGA591200.054.67804316
ACGTCTG598350.054.3167515
GCACACG628850.053.9295911
CACGTCT609100.053.81167614
CACACGT632450.053.43446712
CGGAAGA729650.051.7746854
TCGGAAG743850.051.006173
ATCGGAA761150.050.6919482
GATCGGA764950.049.885711
GAACTCC646900.048.3415821
AGAGCAC749750.047.7885368
AGCACAC736700.047.46696510
CTGAACT680450.047.149319
TCTGAAC720350.044.9748218
TGAACTC713000.044.70912620
AACTCCA699600.044.38950322
GTCTGAA747550.043.83174517
GAGCACA811150.043.6879659
GAAGAGC846450.043.2736636
Produced by FastQC (version 0.11.5)