FastQCFastQC Report
Mon 24 Sep 2018
HHJCJBGX7_n01_nk04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHJCJBGX7_n01_nk04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29979503
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC9490703.1657295986527862TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGC627970.20946644779268023TruSeq Adapter, Index 2 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1067600.067.2419543
CTCGTAT1082500.067.2345242
TCTCGTA1085850.067.1143441
CGTATGC1125300.066.8887944
CGATGTA1322450.066.7889634
TATCTCG1138900.066.6156639
ATGCCGT1099850.066.5206547
TATGCCG1139100.066.4439746
CACCGAT1343000.066.30894531
ACCGATG1339550.066.2993332
CCGATGT1336100.066.20071433
ATCGGAA1380900.066.1053162
ATCTCGT1133600.065.9392440
CGTCTGA1373550.065.9058516
GATCGGA1380000.065.900981
CCGTCTT1078400.065.8148150
ACGTCTG1376950.065.7657715
ACACGTC1381150.065.6815913
GTATGCC1166800.065.5084945
TGCCGTC1111000.065.2102948
Produced by FastQC (version 0.11.5)