FastQCFastQC Report
Mon 24 Sep 2018
HHJCJBGX7_n01_nk03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHJCJBGX7_n01_nk03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34548756
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC2894150.8377002054719423TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC1096310.31732256871998515TruSeq Adapter, Index 5 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG468500.065.5098343
CTCGTAT347150.065.2409442
CGTATGC499800.065.0903544
TCTCGTA349550.064.5528741
ATGCCGT494250.064.20595647
TATGCCG514300.063.4794246
CCGTCTT493400.062.32267450
TGCCGTC505550.061.98125548
GCCGTCT489400.061.716749
GTCGTAT141900.061.6875142
ATCTCGT369200.061.36337340
CGTCTTC492550.060.58275251
CGTCTGA633600.060.27001616
ACACGTC636300.060.1675113
GTATGCC548650.060.00283445
ACGTCTG640400.059.73380315
TGTCGTA146150.059.4388241
ATCGGAA660300.058.2360422
CACGTCT660200.058.0001514
GATCGGA661600.057.8269581
Produced by FastQC (version 0.11.5)