FastQCFastQC Report
Mon 24 Sep 2018
HHJCJBGX7_n01_nk02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHJCJBGX7_n01_nk02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27507815
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC9679913.5189672462171204TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1134850.064.965842
TCGTATG1179050.064.78638543
TCTCGTA1127000.064.7786841
ATGCCGT1179050.064.4681847
CGTATGC1184800.064.46252444
TATGCCG1185400.064.2940846
CCGTCTT1178350.064.0964750
AATCTCG1129300.064.0333339
ATCTCGT1132650.064.01366440
GTATGCC1196000.063.8354545
TGCCGTC1200250.063.22764648
CGTCTTC1190800.063.22059251
GCTTGAA1189100.063.15192859
GCCGTCT1196950.063.04804249
ACCAATC1133100.062.9667636
CGTCTGA1242000.062.93568416
ACGTCTG1246350.062.76646415
GACCAAT1176200.062.7484835
TGCTTGA1192150.062.71732758
CCAATCT1153000.062.3510737
Produced by FastQC (version 0.11.5)