FastQCFastQC Report
Mon 24 Sep 2018
HHJCJBGX7_n01_mb141.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHJCJBGX7_n01_mb141.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31558293
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGC8694262.754984244553405TruSeq Adapter, Index 14 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATGTCGTATGC780650.2473676253655418TruSeq Adapter, Index 14 (97% over 41bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA1014000.065.7946941
TCGTATG1129000.065.72681443
CTCGTAT1034450.065.71529442
CGTATGC1160750.065.56875644
ATGCCGT1150800.065.4484447
TATGCCG1170400.065.2345746
TGCCGTC1145850.065.0989148
GCCGTCT1118850.065.0339649
CCGTCTT1125250.065.0184550
ATCTCGT1025100.064.9627540
CGTCTTC1113000.064.64918551
CATCTCG1030600.064.589139
GTATGCC1206900.063.43859545
CGTCTGA1322550.062.92219516
ACGTCTG1334150.062.43515815
ACACGTC1348150.062.0613113
CACGTCT1360800.061.36626414
GCTTGAA1177600.060.93904559
CACACGT1391150.060.20553612
GCACACG1399550.059.89660611
Produced by FastQC (version 0.11.5)