FastQCFastQC Report
Mon 24 Sep 2018
HHJCJBGX7_n01_mb140.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHJCJBGX7_n01_mb140.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34120146
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGC10673113.128096227958696TruSeq Adapter, Index 13 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA1251250.064.7488541
CTCGTAT1256150.064.6996742
TCGTATG1304450.064.60612543
CGTATGC1310550.064.4412444
AATCTCG1248100.064.3567539
ATGCCGT1308000.064.3474647
ATCTCGT1258850.064.13792440
TATGCCG1317350.064.0846946
TGCCGTC1310350.064.0795248
GCCGTCT1302600.064.0119749
CCGTCTT1304450.063.9909550
CGTCTTC1307200.063.5591351
CGTCTGA1397700.063.10946316
ACGTCTG1407750.062.71850215
ACACGTC1422600.062.4108213
GTATGCC1360000.062.17276845
CACGTCT1430250.061.881414
CACACGT1473350.060.28217312
GCTTGAA1375550.060.19211659
GCACACG1482650.059.9442711
Produced by FastQC (version 0.11.5)