FastQCFastQC Report
Mon 24 Sep 2018
HHJCJBGX7_n01_mb139.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHJCJBGX7_n01_mb139.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32722549
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC1621960.49567043203144107TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT206850.059.54205342
TCGTATG208950.059.5131443
TCTCGTA205700.059.2114541
CGTATGC213000.058.89075544
ATGCCGT214950.058.07979247
TATGCCG218900.057.41539446
TGCCGTC219000.057.06946648
ACGGCTA221350.056.59102232
GCCGTCT218650.056.56855849
CGGCTAC220250.056.4922833
CCGTCTT221500.055.87222350
ATCTCGT219800.055.18998340
CGTCTTC229900.053.55675551
CATCTCG229300.052.8118639
TCACGGC242000.052.02216730
GTCACGG251650.050.40564729
CACGGCT249700.050.32015231
AGTCACG259550.049.1547828
GTATGCC264600.047.75029845
GCTTGAA309250.040.1993859
Produced by FastQC (version 0.11.5)