FastQCFastQC Report
Mon 24 Sep 2018
HHJCJBGX7_n01_mb138.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHJCJBGX7_n01_mb138.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40173603
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC10880692.708417763773889TruSeq Adapter, Index 10 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATGC1598760.39796281155065927TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTTTGC515510.12832057906282393TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATTC477900.11895871027550106TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGG468680.11666367091843866TruSeq Adapter, Index 10 (97% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1335800.066.0560442
TCTCGTA1346300.065.9805441
TATCTCG1491200.065.9530539
CGTATGC1410150.065.8788444
TCGTATG1286750.065.7941243
TATGCCG1443100.065.6431546
ATGCCGT1335600.065.5336447
TCGCGTA227700.065.480641
TATCGCG319900.065.43709639
ATCTCGT1444700.065.3524240
TGCCGTC1332150.065.19556448
CGCGTAT226650.065.1659442
CCGTCTT1301700.064.892450
GCCGTCT1250200.064.7520549
ATCGCGT270800.064.5965540
CGTCTTC1259500.064.4323351
GCTTATC2063550.064.37897536
GTATGCC1525300.063.58566345
CTTATCG417000.063.5371237
TAGCTTA2165000.063.22757734
Produced by FastQC (version 0.11.5)