FastQCFastQC Report
Mon 24 Sep 2018
HHJCJBGX7_n01_mb137.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHJCJBGX7_n01_mb137.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43933140
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC11436642.6031920322562874TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1363250.063.49347342
TCTCGTA1349450.063.37780841
TCGTATG1399000.063.2467843
CGTATGC1404200.063.09189244
GATCTCG1336750.062.9926339
ATGCCGT1403550.062.94627847
CGATCAG1400800.062.89138833
TATGCCG1409200.062.7610346
ACGATCA1413400.062.58338532
GCCGTCT1408500.062.54110749
TGCCGTC1411450.062.53692648
CCGTCTT1410650.062.4827950
ATCTCGT1350900.062.43645540
CACGATC1423250.062.18229731
TCACGAT1429300.061.97960330
GTCACGA1430650.061.97881729
CGTCTTC1421700.061.9651551
AGTCACG1463700.060.8447228
GTATGCC1467300.060.33560645
CGTCTGA1566500.057.77997616
Produced by FastQC (version 0.11.5)