FastQCFastQC Report
Mon 24 Sep 2018
HHJCJBGX7_n01_mb136.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHJCJBGX7_n01_mb136.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39622136
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC5351141.3505430398805354TruSeq Adapter, Index 8 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATGC990500.249986522685198TruSeq Adapter, Index 8 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA147300.065.53142541
CGCGTAT145350.065.27869442
AATCTCG808300.065.25732439
ATCGCGT179800.064.3141140
TCTCGTA714000.063.69462241
CTCGTAT707150.063.6828942
AATCGCG236750.063.44968439
TCGTATG676150.063.0517243
CGTATGC750700.062.9955244
ATCTCGT789550.062.58643340
TATGCCG774750.062.5218846
ATGCCGT701100.062.49527747
TGCCGTC700400.061.71800248
GAATCGC250100.061.5883438
TGAATCG321500.061.37767437
GCCGTCT648150.061.1315949
CCGTCTT688000.061.1006950
CGTCTTC662900.059.98240751
TTGAATC1303350.059.8357236
GTATGCC845900.058.91385745
Produced by FastQC (version 0.11.5)