FastQCFastQC Report
Mon 24 Sep 2018
HHJCJBGX7_n01_mb135.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHJCJBGX7_n01_mb135.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29593784
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC3697611.2494549531077201TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA436850.064.6235541
TCGTATG447650.064.5496443
CTCGTAT442050.064.42518642
CGTATGC467500.064.2716244
ATGCCGT464150.063.79314447
TGCCGTC464050.063.39929648
TATGCCG476050.063.38216846
GCCGTCT450750.063.1113949
ATCTCGT455350.062.98934640
CCGTCTT459700.062.7276650
CATCTCG462450.062.52934639
CGTCTTC456300.061.56129551
GTATGCC512400.059.4456945
CGTCTGA586150.059.42765416
ACGTCTG598000.058.32026715
TCGCGTA23700.058.1852441
ACACGTC609700.057.43025613
CGCGTAT25700.057.06155842
CACGTCT618250.056.51186814
CTTAGGC600700.056.4876733
Produced by FastQC (version 0.11.5)