FastQCFastQC Report
Mon 24 Sep 2018
HHJCJBGX7_n01_mb134.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHJCJBGX7_n01_mb134.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41554554
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC4690621.1287860290835994TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT574800.063.50808742
TCGTATG592800.063.33905443
TCTCGTA566150.063.3162741
CGTATGC595500.063.21033544
GATCTCG561050.062.7130239
ATGCCGT598350.062.69268447
CGATCTC561900.062.4688138
TATGCCG605350.062.12383346
TGCCGTC605800.061.90999648
ACGATCT565050.061.8980237
CACGATC565000.061.87872736
ATCTCGT573950.061.4799740
GCCGTCT607500.061.4601449
TCACGAT584950.061.3480935
CCGTCTT610800.061.19662550
ATCACGA598900.061.1812234
CGTCTTC618700.060.3870151
CATCACG623950.059.85237533
GTATGCC657200.057.2918645
GCTTGAA722550.051.6785859
Produced by FastQC (version 0.11.5)