FastQCFastQC Report
Mon 24 Sep 2018
HHJCJBGX7_n01_mb133.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHJCJBGX7_n01_mb133.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45154973
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC6587461.4588559271201424TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT765650.065.1308242
TCTCGTA767300.064.9405941
TCGTATG820300.064.8961543
CGTATGC832400.064.6085944
AATCTCG775700.064.435639
ATGCCGT824500.064.4251347
TATGCCG838550.064.0511946
TGCCGTC824850.063.98600848
GCCGTCT813800.063.62058649
CCGTCTT821900.063.525650
ATCTCGT788900.063.35294740
CGTCTTC821700.062.73185351
GTATGCC900050.060.0363845
GTAATCT874550.057.4691437
GCTTGAA928850.055.4535659
CGTCTGA1051550.055.16583616
TGTAATC936200.054.55198336
TAATCTC922850.054.4228638
ACGTCTG1077950.053.82758315
CCTTGTA1048700.053.71933433
Produced by FastQC (version 0.11.5)