FastQCFastQC Report
Mon 24 Sep 2018
HHJCJBGX7_n01_mb132.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHJCJBGX7_n01_mb132.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39019234
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC8993512.304891479930129TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTATGC918860.23548898986586975TruSeq Adapter, Index 7 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1049450.067.18780542
TCTCGTA1051150.067.0895741
TCGTATG1052600.067.0032843
CGTATGC1130450.066.9522844
ATGCCGT1089100.066.6596947
TATGCCG1143150.066.5543546
TGCCGTC1088000.066.3118648
GCCGTCT1029650.066.1743349
ATCTCGT1117450.066.1625140
TCGCGTA126350.066.01079641
CCGTCTT1048100.065.9877750
CATCTCG1153750.065.67947439
CGCGTAT128400.065.5288942
CGTCTTC1025750.065.31010451
ATCGCGT148800.064.4718440
CATCGCG172300.063.84435339
GTATGCC1207200.063.8262845
GATCATC1451450.063.0698236
ACCAGAT1600950.060.7762332
AGATCAT1558250.060.7731135
Produced by FastQC (version 0.11.5)