FastQCFastQC Report
Tue 4 Jun 2019
HHHCYBGXB_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHHCYBGXB_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences518059130
Sequences flagged as poor quality0
Sequence length92
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7791360.15039518751459896No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGACCCCAGGTCAGGCGGGA6014100.11608906496831742No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGACCCCAGGTCAGGCGGG5862800.11316854892606563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT30217700.073.738111
AGCAGTG31730000.069.964482
GCAGTGG36152550.061.2674263
AGTGGTA39235700.056.703355
CAGTGGT39596100.055.972974
GTGGTAT42677800.052.0985376
GGTATCA43415550.051.0554858
TGGTATC43594150.050.8652887
GTATCAA44257950.050.090089
CAACGCA42988900.028.68999912-13
ACGCAGA43081900.028.6678314-15
ATCAACG43948250.028.20432310-11
GCAGAGT43981800.028.112816-17
AGTACAT44101050.027.90179820-21
TACATGG43750450.027.86377722-23
AGAGTAC44544450.027.76381518-19
CATGGGG21905350.026.86600124-25
GGGCGAC2484350.026.05635328-29
GCGACCC2320150.025.80441330-31
GTACATG43006450.025.60781322-23
Produced by FastQC (version 0.11.5)