FastQCFastQC Report
Tue 4 Jun 2019
HHHCYBGXB_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHHCYBGXB_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences518059130
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCATCA7771978215.00210641978262No Hit
TCCGGAAG6502602612.551854071175233No Hit
CCTGTGCG6209590911.986258981672613No Hit
GGACACGT6008193211.597504709549275No Hit
GTAATCTT5747244711.093800624650703No Hit
ATGAGAAA5638856210.884580298777863No Hit
TACTCTTC5542155310.697920331989902No Hit
AGTTCGGC506694059.780621953327993No Hit
GGGGGGGG140253592.7072892239154247No Hit
GGCCACGT9039630.1744903134898906TruSeq Adapter, Index 20 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)