FastQCFastQC Report
Tue 4 Jun 2019
HHHCYBGXB_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHHCYBGXB_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences518059130
Sequences flagged as poor quality0
Sequence length29
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGAAG1610600.018.7284561
GCGAGTG1674400.018.3549865
GGAGGAA1452600.017.9425776
CAACTCC2106700.017.7756968
TGCACCC1621500.017.75070210
CAAAAGA850400.017.6183664
GGGATCC1484900.017.5318531
GTGCGAG1662550.017.4239373
GTGCACC1661750.017.3732479
GGAAGCA1417450.017.3155963
AAACGAA1185450.017.0860841
TGCCGAC1120250.016.96863610
GACGGAA875200.016.6911189
AACTCCC1329450.016.686659
AACGGGA1101850.016.6670881
GAGTGCA2128850.016.6152977
TAGACGA842350.016.6138977
ACTACGA1173850.016.4552461
AGGTAGG1864400.016.4535351
AGTGCAC2126500.016.3998668
Produced by FastQC (version 0.11.5)