FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40144250
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9826172.447715426244107No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGTA152150.027.6399672
CGAAAGC115200.026.7361343
CAGGTAC161150.026.684723
GGATAGC165100.026.2687875
GTATAGG147600.025.321351
TATACTG233800.025.1061215
ACTGATC239050.024.754168
TATAGCG32600.024.4422282
GACAGTA180450.024.0819577
TTGGACA218750.022.7550854
GGACAGT249750.021.0882726
TATGGAT239450.019.6580522
GTATGGA308750.019.1623271
CTGATCC263550.018.6234159
TGGATAG245250.018.3024084
CTATACT154100.017.2401434
CCTATAC154500.017.2233313
TCTATAC288850.017.0505943
CCTTATA224300.016.545252
GTCGGTA94600.016.033493145
Produced by FastQC (version 0.11.5)