FastQCFastQC Report
Thu 10 Sep 2020
HHH35BGXF_n02_pDZPB2_amp.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_pDZPB2_amp.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3966
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA160.4034291477559254No Hit
CTCATAGACTTTGGCACTCCTTCCGTAGAAGGCCCTCCTTTCAGTCCGTA100.2521432173474533No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA100.2521432173474533No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT90.22692889561270801No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT90.22692889561270801No Hit
CCTTTCAGTCCGTATTTAAAGCGACGGTAAATGCATTTGAAAAAAAGACG80.2017145738779627No Hit
GCACTGGAGCTAGGATGAGTCCCAATGGTTCTCATCGCTTGCACCATTTG80.2017145738779627No Hit
TGTCTAGCCTGACTAGCAACCTCCATGGCCTCTGCTGCTTGCTCACTCGA80.2017145738779627No Hit
GCTATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGT80.2017145738779627No Hit
ATCTACAGTATCACTATTCACGCCACAAAAAGAAATGCTGCTCGCACTAG80.2017145738779627No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT80.2017145738779627No Hit
ATATAGCGGTATTCCCTCTCCTTCATGACTTGGGTCCTCAATGCTTAATT70.17650025214321735No Hit
ACTCAGCACCGTCTGGCCAAGACCAATCTACAGTATCACTATTCACGCCA70.17650025214321735No Hit
GTCCCAATGGTTCTCATCGCTTGCACCATTTGTCTAGCCTGACTAGCAAC70.17650025214321735No Hit
CTATTAAGAGCGGTCGGATTTTTTCAATCTTCTTTCTTGTTGAATCATTG70.17650025214321735No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA70.17650025214321735No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG70.17650025214321735No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA70.17650025214321735No Hit
CCGCTATATGATGCAATCAAATGCATGAGAACATTCTTTGGATGGAAGGA70.17650025214321735No Hit
ATCCAGCCATTTGCTCCATAGCCTTAGCTGTAGTGCTGGCTAAAACCATT70.17650025214321735No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTTTGGCACTCCTTCCG70.17650025214321735No Hit
ATCCACAGCACTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTTTG60.15128593040847202No Hit
GTAGTAAACAGTATTTGCAACACTACAGGGGCTGAGAAACCAAAGTTTCT60.15128593040847202No Hit
TATTTAAAGCGACGGTAAATGCATTTGAAAAAAAGACGATCAAGAATCCA60.15128593040847202No Hit
CCATTTACACAGGCACATTCAGACTCTTGTGTCCTCAATATTTTCTTCCT60.15128593040847202No Hit
GGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTACT60.15128593040847202No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC60.15128593040847202No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT60.15128593040847202No Hit
GTATTAGGCGTCTCCATCCTGAATCTTGGACAAAAGAGATACACCAAGAC60.15128593040847202No Hit
GCCTCATACAGTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCG60.15128593040847202No Hit
TTTTAATACAGCCACTGCTCCATTATCTGGACCTGAAATTCCGATTGTTA60.15128593040847202No Hit
CAATAGAAGTGTTCAGATCAAATGGCCTCACGGCCAATGAGTCTGGAAGG60.15128593040847202No Hit
GTTTTTTACTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCAC60.15128593040847202No Hit
CCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATAGGCAAATGGTG60.15128593040847202No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG60.15128593040847202No Hit
GTTCTACACTTGCTTATCATTGGAATTAATTGGAAATCATCCATTGCTGC50.12607160867372666No Hit
GCTATACCCTGACCAATCAGTCATTGCCACAACATCTTGCCTAACAGAGA50.12607160867372666No Hit
CTCCAACATGTATGCAACCATCAAAGGAGAAATTTTGCAATCCTGGAGTT50.12607160867372666No Hit
AGATAATGGAGCAGTGGCTGTATTAAAATACAACGGCATAATAACTGAAA50.12607160867372666No Hit
CTTCTAACCGAGGTCGAAACGTACGTACTCTCTATCATCCCGTCAGGCCC50.12607160867372666No Hit
CACATATACTATCTGGAAAAGGCCAATAAAATTAAATCTGAGAAAACACA50.12607160867372666No Hit
GCCCATCACTCGGGCCATCAGTCATTATAGTAAAACATGAACCATTTACA50.12607160867372666No Hit
ATGTTAAGCACTGTATTAGGCGTCTCCATCCTGAATCTTGGACAAAAGAG50.12607160867372666No Hit
ATGCAGGATCAGACCGAGTGATGGTATCACCTCTGGCTGTGACATGGTGG50.12607160867372666No Hit
CCACAATGTCAAGTGCAAGATCCCAATGATATTTGCGGCAATAGCGAGAG50.12607160867372666No Hit
CTCCAATGGGCCATGTTTCTGATTTGTTCTCAAAGAACTCTTTGGTCATG50.12607160867372666No Hit
GTGTATGTTGATGGAGCAAACGGAGTAAAGGGATTTTCATATAGGTATGG50.12607160867372666No Hit
GTTATTCTGCTGGTGCACTTGCCAGTTGTATGGGCCTCATATACAACAGG50.12607160867372666No Hit
GTGTTCACGCTCACCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTT50.12607160867372666No Hit
GTTCTTGAGATAGGAGATATGCTTATAAGAAGTGCCATAGGCCAGGTTTC50.12607160867372666No Hit
GAAGAACACTGATCTTGAGGTTCTCATGGAATGGCTAAAGACAAGACCAA50.12607160867372666No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG50.12607160867372666No Hit
GGTAAATGCATTTGAAAAAAAGACGATCAAGAATCCACAATGTCAAGTGC50.12607160867372666No Hit
AATATGCACTCACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCA50.12607160867372666No Hit
ACTTAGAACATTTCTGAACCATTCGGGCTGATTTCTTGTCATATATGTGA50.12607160867372666No Hit
GGATGTAATCATGGAAGTTGTTTTCCCTAACGAAGTGGGAGCCAGGATAC50.12607160867372666No Hit
CAATGATGCAGTCCCCTCTATTAAGAGCGGTCGGATTTTTTCAATCTTCT50.12607160867372666No Hit
GGTATGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGA50.12607160867372666No Hit
ATTCAAGATTTGAATCGGTTGCTTGGTCAGCAAGTGCATGTCATGATGGC40.10085728693898136No Hit
ATAACAAATACAGTTCATTATCCAAAAATCTACAAAACTTATTTTGAAAG40.10085728693898136No Hit
CCTTTGGCCCTGTCCATTTTAGAAACCAAGTCAAAATACGTCGGAGAGTT40.10085728693898136No Hit
GTCTCTGTCCATCCATTAGGATCCCAAATCATCTCAAACCCATGTCTGGA40.10085728693898136No Hit
CCTTTGGGGGACTCTCCAATGGGCCATGTTTCTGATTTGTTCTCAAAGAA40.10085728693898136No Hit
CCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGG40.10085728693898136No Hit
CACCATTACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCA40.10085728693898136No Hit
ATATGATAGTGATGAACCAGAATTGAGGTCGCTAGCAAGTTGGATTCAGA40.10085728693898136No Hit
CGTTTATGCAACCCACTGAACCCATTTGTCAGCCATAAAGAAATTGAATC40.10085728693898136No Hit
GGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGTATGTGCAACC40.10085728693898136No Hit
ACCCAATGTTGTTAAACCACACGAAAAGGGAATAAATCCAAATTATCTTC40.10085728693898136No Hit
GAGTATATGTGAGAAACTTGAACAATCAGGGTTGCCAGTTGGAGGCAATG40.10085728693898136No Hit
GCGTAGACGCTTTGTCCAAAATGCCCTTAATGGGAACGGGGATCCAAATA40.10085728693898136No Hit
AAACAAGGTAGTTTTTTACTCCAGCTCTATGCTGACAAAATGACCATCGT40.10085728693898136No Hit
ATTCTAGCATTTACTTCTTTGGACATTTGAGACAGCTTGCCCTCAATGTA40.10085728693898136No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40.10085728693898136No Hit
GCTTAGTGCTGGCCCATATCTCTTGTCTTCTTTGCCCAGAATGAGGAATC40.10085728693898136No Hit
CTCTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACT40.10085728693898136No Hit
GTATAGCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTCCCAGT40.10085728693898136No Hit
GTTCAGAAATGTTCTAAGTATTGCTCCAATAATGTTCTCAAACAAAATGG40.10085728693898136No Hit
GATATATGCAAGGCTGCAATGGGACTGAGAATTAGCTCATCCTTCAGTTT40.10085728693898136No Hit
CATGAGAACAGAATGGTTTTAGCCAGCACTACAGCTAAGGCTATGGAGCA40.10085728693898136No Hit
TTTCTGAACCATTCGGGCTGATTTCTTGTCATATATGTGATCATGGCCAA40.10085728693898136No Hit
GTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGAGTG40.10085728693898136No Hit
CAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGA40.10085728693898136No Hit
CTACAGCTAAGGCTATGGAGCAAATGGCTGGATCGAGTGAGCAAGCAGCA40.10085728693898136No Hit
CCTCATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCC40.10085728693898136No Hit
CCATTATCTGGACCTGAAATTCCGATTGTTAGCCAGCCCATGCCATCATG40.10085728693898136No Hit
CAATTATTGCTTCGGCAATCGACTGTTCGTCTCTCCCACTCACTATCAGC40.10085728693898136No Hit
GCCTAATATTGCAAATAGGGAATATAATCTCAATATGGATTAGCCATTCA40.10085728693898136No Hit
CCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGAAGCTCCGTCCCCGT40.10085728693898136No Hit
GTATTGGAGTTACTGTCATCAAAAACAATATGATAAACAATGATCTTGGT40.10085728693898136No Hit
CATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGG40.10085728693898136No Hit
CCCTTAATGGGAACGGGGATCCAAATAACATGGACAAAGCAGTTAAACTG40.10085728693898136No Hit
ATTACAAGGAGAATAGATTCATCGAAATTGGAGTAACAAGGAGAGAAGTT40.10085728693898136No Hit
AATCAGACATGAGAACAGAATGGTTTTAGCCAGCACTACAGCTAAGGCTA40.10085728693898136No Hit
GTATTGATGTACACCCCCTTCATTATGTATTCTGTGGCTCTGCAGTGAGA40.10085728693898136No Hit
CTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAG40.10085728693898136No Hit
GGCCTGGACAGTAGTAAACAGTATTTGCAACACTACAGGGGCTGAGAAAC40.10085728693898136No Hit
CTTCATATCCCTCATGCACTCTTATCTTCAATGTTTGAAGATTGCCCGTA40.10085728693898136No Hit
GCACTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTTTGGCACTCC40.10085728693898136No Hit
GTGTAGAACTAAGGAGGGAAGGCGAAAGACCAACTTGTATGGTTTCATCA40.10085728693898136No Hit
GAGTTACTGTCATCAAAAACAATATGATAAACAATGATCTTGGTCCAGCA40.10085728693898136No Hit
CTCATACAGTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCT40.10085728693898136No Hit
ATTCAGACTCTTGTGTCCTCAATATTTTCTTCCTCCAACTTTTTATGGTT40.10085728693898136No Hit
GTTAAACTGTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAA40.10085728693898136No Hit
ATTACATCCTGTGCCTCCTTGGCACTGAGATCTGCATGACCAGGATTTAT40.10085728693898136No Hit
GTGCTGGAGTACTCATCTACACCCATTTTGCTGATTCTCACTCCTCTCAT40.10085728693898136No Hit
CAGTTATTCTGCTGGTGCACTTGCCAGTTGTATGGGCCTCATATACAACA40.10085728693898136No Hit
GTATTTTAATACAGCCACTGCTCCATTATCTGGACCTGAAATTCCGATTG40.10085728693898136No Hit
ATCAAAACCTGGATTATCAAATAGGATACATCTGCAGTGGGGTTTTCGGT40.10085728693898136No Hit
CTCTTTGACAGAGGACTCAGCTTCAATCATACTCTCAATTTGTTGAAGTG40.10085728693898136No Hit
ATGCAGTGATGATGCCAGCACATGGTCCAGCCAAAAACATGGAGTATGAT40.10085728693898136No Hit
GCTCTGCAGTGAGACACCTCTGATGTGAAATAATTCCTTCTCATGCTTGC40.10085728693898136No Hit
GTTTTGGGGTGTCTGGGATCAACGAGTCAGCGGACATGAGTATTGGAGTT40.10085728693898136No Hit
TTCCTGAAGTCTGCCTAAAATGGGAATTGATGGATGAGGATTACCAGGGG40.10085728693898136No Hit
GAGTTTAACAAGGCATGCGAACTGACAGATTCAAGCTGGATAGAGCTCGA40.10085728693898136No Hit
TCTTTGGACATTTGAGACAGCTTGCCCTCAATGTAGCCGTTCGGTTCGAA40.10085728693898136No Hit
ACTGTCAACAGGACACATCAGTACTCAGAAAAGGGAAGATGGACAACAAA40.10085728693898136No Hit
CATTAAGGCCCTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCT40.10085728693898136No Hit
CGCTCACCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTGTCCAA40.10085728693898136No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT40.10085728693898136No Hit
GTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGATTCAGCTGAAA40.10085728693898136No Hit
CCACAGCACTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTTTGGC40.10085728693898136No Hit
GGCACATTCAGACTCTTGTGTCCTCAATATTTTCTTCCTCCAACTTTTTA40.10085728693898136No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGGA100.00682755145.02
GTAGAAA100.00682755145.01
AAGGTCG100.00682755145.09
CAAGGTC100.00682755145.08
ACAAGGT100.00682755145.07
Produced by FastQC (version 0.11.5)