FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_PB2_nonamp_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_PB2_nonamp_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2262951
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTTGTTATTGGTCCATTCCTATTCCACCATGTCACAGCCAGAGGTGA44990.1988111982981514No Hit
AACAAATACAGTTCATTATCCAAAAATCTACAAAACTTATTTTGAAAGAG44930.19854605778030546No Hit
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG44680.197441305622614No Hit
GTATAGCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTCCCAGT41850.18493551119754692No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT41630.18396332929877846No Hit
GGATGTAATCATGGAAGTTGTTTTCCCTAACGAAGTGGGAGCCAGGATAC41500.1833888581767789No Hit
TCCTTAGGCAGAACCCAACAGAAGAGCAAGCCGTGGATATATGCAAGGCT41020.18126773403401134No Hit
GTATTGGCCTCTAATGGCCTTAGGTACTAAAGACTGAAATGGTTCAAATT39170.1730925680670947No Hit
ATTACATCCTGTGCCTCCTTGGCACTGAGATCTGCATGACCAGGATTTAT37990.1678781378827911No Hit
CCCATATGTCCTTCCGAGTGAGAGACACAAAAAATTCCAACACACTATTG36360.16067515381464292No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG35430.15656547578803076No Hit
GTCGATAGGTTCAACTCCCCAATTTTGAAAAAGCACTTTCGCATCCTTCT35260.1558142443208006No Hit
CTTTATAGCCAACCTTTGTTCATGGCAGCCAGCATATGGGCATATGTTGC35230.1556816740618776No Hit
ATCCCAATCATTCCCATCACATTGTCGATAGGTTCAACTCCCCAATTTTG34010.1502904835323434No Hit
CTCCAACATGTATGCAACCATCAAAGGAGAAATTTTGCAATCCTGGAGTT33930.14993696284188213No Hit
ATGTACTACTGTCTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAGAAA31900.14096637532142764No Hit
ATCTTATCATGTCTGGATCCGCTGCATTAATGAATCAGGGGATAACGCAG31790.1404802843720434No Hit
GTGCTGGAGTACTCATCTACACCCATTTTGCTGATTCTCACTCCTCTCAT30800.13610546582758531No Hit
GCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAA30570.13508909384250917No Hit
TTGCAATAGTGTGTTGGAATTTTTTGTGTCTCTCACTCGGAAGGACATAT30520.1348681434109709No Hit
ATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAA30400.134337862375279No Hit
GAACAGAGAAACTGACAATAACTTACTCATCGTCAATGATGTGGGAGATT30060.13283539944081865No Hit
GATATATGCAAGGCTGCAATGGGACTGAGAATTAGCTCATCCTTCAGTTT29920.13221673823251145No Hit
CTAATGGCCTTAGGTACTAAAGACTGAAATGGTTCAAATTCCATTTTATT29710.13128874642005064No Hit
ATTCTAGACGGGCCGGCCCCTGCGTGTGGCACGGGCGGCCGGGAGGGCGT29660.13106779598851234No Hit
GCCTAAGGATGTCTACCATCCTAATTCCACCAATCTGTGTGCTGTGGCAC29500.13036075460758983No Hit
CCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACC29410.1299630438308209No Hit
CTATTGCAATGAAAATAAATTTCCTTTATTAGCCAGAAGTCAGATGCTCA29370.1297862834855903No Hit
ATGCTATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTACCTAA29360.1297420933992826No Hit
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCT29020.12823963046482226No Hit
CCACTACCCTCTCCGTGCTGGAGTACTCATCTACACCCATTTTGCTGATT28920.12779772960174568No Hit
GATATGAAGAGTTCACAATGGTTGGGAGAAGAGCAACAGCCATACTCAGA28900.12770934942913037No Hit
GTATGCAACCATCAAAGGAGAAATTTTGCAATCCTGGAGTTCTTCTTTCT28780.12717906839343848No Hit
GAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTT28740.12700230804820786No Hit
GTCTGATCCTGCATCATTCATTTTACTCCATAAAGTTTGTCCTTGCTCAT28270.12492537399174794No Hit
CTCATTGACATCTCGATGCTTGGAGTCATGTCTGGCAATATCCCAATCAT28240.12479280373282498No Hit
CTTCATATCCCTCATGCACTCTTATCTTCAATGTTTGAAGATTGCCCGTA28190.12457185330128667No Hit
GTATCAAATGTCCCAAGCACATCCCTCATTTGTTGGAACAGAGTTCTTAC28140.1243509028697484No Hit
ATCCTGCATCATTCATTTTACTCCATAAAGTTTGTCCTTGCTCATTTCTC27890.12324615071205695No Hit
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA26740.11816429078667634No Hit
GAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGG26090.11529193517667859No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC25920.11454070370944841No Hit
GATAAAAGCAGTCAGAGGTGATCTGAATTTCGTCAATAGGGCGAATCAGC25840.11418718301898716No Hit
ATTGAATCCTATGCATCAACTTTTAAGACATTTTCAGAAGGATGCGAAAG25690.11352433172437228No Hit
CAATAATTGTGGCCATGGTATTTTCACAAGAGGATTGTATGATAAAAGCA25540.11286148042975742No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGAT25330.11193348861729663No Hit
GTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATT25050.1106961662006822No Hit
GTTTTATTATCTGTGCGGTATCAAATGTCCCAAGCACATCCCTCATTTGT24860.10985655456083672No Hit
GCATTAATGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGC24830.10972398430191374No Hit
GGCTAAAGCATGGAACCTTTGGCCCTGTCCATTTTAGAAACCAAGTCAAA24770.10945884378406778No Hit
TTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGT24690.10910532309360654No Hit
TTCTCACTCCTCTCATTGACATCTCGATGCTTGGAGTCATGTCTGGCAAT24630.10884018257576059No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA24590.10866342223052995No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA24270.10724933946868491No Hit
GTATTTTCACAAGAGGATTGTATGATAAAAGCAGTCAGAGGTGATCTGAA24220.10702838903714662No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC23860.10543754593007096No Hit
AACTTACTCATCGTCAATGATGTGGGAGATTAATGGTCCTGAATCAGTGT23620.10437698385868718No Hit
TTTCAGAAGGATGCGAAAGTGCTTTTTCAAAATTGGGGAGTTGAACCTAT23530.10397927308191826No Hit
GTCCTGGGGTTGACCAGAGGGCCCCGGGCGCTCCGTGTGTGGCTGCGATG23410.10344899204622637No Hit
CTTCTGAAAATGTCTTAAAAGTTGATGCATAGGATTCAATCGCTGATTCG23250.10274195066530384No Hit
CTCCTGGAGTATACATCTGTTCCCAGCATGTTCCTTGAGTCAAATGCAAC23050.10185814893915068No Hit
GGTCAATACCTATCAATGGATCATCAGAAACTGGGAAACTGTTAAAATTC22880.10110691747192051No Hit
GTATACATCTGTTCCCAGCATGTTCCTTGAGTCAAATGCAACACTTCAAT22700.10031149591838268No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT100.0070934086144.9866145
CCGTTCC1300.089.222534145
GACGGAC1150.069.34142145
TCGACTA350.00356839162.13712145
TTTCGCG1051.6370905E-1162.137115145
CCGACGC2200.059.312702145
TCTGACC1800.056.383686145
TGGTATA1051.1114025E-955.23299145
GCCGACC1850.054.8598145
GTCGGCC553.2213406E-452.722404145
TGACGCA1258.913048E-1152.195183145
AGGACCC858.935549E-751.171745145
CATGTTA1600.049.839146145
GGACGGG752.5340565E-548.3898161
CGGGTTC450.00964421448.3288733
GAGTGTT450.00964421448.328873145
GCGTTAA450.00964421448.328873145
CCGTATA901.3251665E-648.328873145
TCTCGGG901.3251665E-648.328873145
TAGTAGG450.00964421448.328873145
Produced by FastQC (version 0.11.5)