FastQCFastQC Report
Thu 10 Sep 2020
HHH35BGXF_n02_Chang_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_Chang_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2224712
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTTGTTATTGGTCCATTCCTATTCCACCATGTCACAGCCAGAGGTGA44580.20038548809913373No Hit
AACAAATACAGTTCATTATCCAAAAATCTACAAAACTTATTTTGAAAGAG44510.20007084063015795No Hit
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG44360.19939659605378132No Hit
GTATAGCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTCCCAGT41560.18681069729475097No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT41250.1854172585035726No Hit
GGATGTAATCATGGAAGTTGTTTTCCCTAACGAAGTGGGAGCCAGGATAC41160.1850127117577466No Hit
TCCTTAGGCAGAACCCAACAGAAGAGCAAGCCGTGGATATATGCAAGGCT40740.18312482694389207No Hit
GTATTGGCCTCTAATGGCCTTAGGTACTAAAGACTGAAATGGTTCAAATT38740.17413489925887038No Hit
ATTACATCCTGTGCCTCCTTGGCACTGAGATCTGCATGACCAGGATTTAT37740.1696399354163595No Hit
CCCATATGTCCTTCCGAGTGAGAGACACAAAAAATTCCAACACACTATTG36050.16204344652251618No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG35060.15759343231843043No Hit
GTCGATAGGTTCAACTCCCCAATTTTGAAAAAGCACTTTCGCATCCTTCT34970.15718888557260446No Hit
CTTTATAGCCAACCTTTGTTCATGGCAGCCAGCATATGGGCATATGTTGC34910.1569191877420538No Hit
ATCCCAATCATTCCCATCACATTGTCGATAGGTTCAACTCCCCAATTTTG33800.15192977787686676No Hit
CTCCAACATGTATGCAACCATCAAAGGAGAAATTTTGCAATCCTGGAGTT33690.15143533185419056No Hit
ATGTACTACTGTCTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAGAAA31650.14226560561546842No Hit
ATCTTATCATGTCTGGATCCGCTGCATTAATGAATCAGGGGATAACGCAG31570.14190600850806756No Hit
GTGCTGGAGTACTCATCTACACCCATTTTGCTGATTCTCACTCCTCTCAT30590.13750094394240694No Hit
GCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAA30250.13597265623595323No Hit
TTGCAATAGTGTGTTGGAATTTTTTGTGTCTCTCACTCGGAAGGACATAT30220.1358378073206779No Hit
ATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAA30120.13538831093642684No Hit
GAACAGAGAAACTGACAATAACTTACTCATCGTCAATGATGTGGGAGATT29790.13390497286839825No Hit
GATATATGCAAGGCTGCAATGGGACTGAGAATTAGCTCATCCTTCAGTTT29730.1336352750378476No Hit
CTAATGGCCTTAGGTACTAAAGACTGAAATGGTTCAAATTCCATTTTATT29440.13233173552351943No Hit
ATTCTAGACGGGCCGGCCCCTGCGTGTGGCACGGGCGGCCGGGAGGGCGT29420.13224183624666924No Hit
GCCTAAGGATGTCTACCATCCTAATTCCACCAATCTGTGTGCTGTGGCAC29240.13143274275501726No Hit
CCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACC29150.1310281960091913No Hit
ATGCTATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTACCTAA29070.13066859890179044No Hit
CTATTGCAATGAAAATAAATTTCCTTTATTAGCCAGAAGTCAGATGCTCA29010.13039890107123978No Hit
CCACTACCCTCTCCGTGCTGGAGTACTCATCTACACCCATTTTGCTGATT28770.12932010974903718No Hit
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCT28760.12927516011061208No Hit
GATATGAAGAGTTCACAATGGTTGGGAGAAGAGCAACAGCCATACTCAGA28740.12918526083376186No Hit
GAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTT28540.1282862680652597No Hit
GTATGCAACCATCAAAGGAGAAATTTTGCAATCCTGGAGTTCTTCTTTCT28490.12806151987313413No Hit
CTCATTGACATCTCGATGCTTGGAGTCATGTCTGGCAATATCCCAATCAT28070.1261736350592796No Hit
CTTCATATCCCTCATGCACTCTTATCTTCAATGTTTGAAGATTGCCCGTA28040.12603878614400427No Hit
GTCTGATCCTGCATCATTCATTTTACTCCATAAAGTTTGTCCTTGCTCAT28030.12599383650557916No Hit
GTATCAAATGTCCCAAGCACATCCCTCATTTGTTGGAACAGAGTTCTTAC27900.12540949120605274No Hit
ATCCTGCATCATTCATTTTACTCCATAAAGTTTGTCCTTGCTCATTTCTC27700.12451049843755058No Hit
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA26510.11916149146496266No Hit
GAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGG25820.11605996641363017No Hit
GATAAAAGCAGTCAGAGGTGATCTGAATTTCGTCAATAGGGCGAATCAGC25640.11525087292197822No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC25560.11489127581457735No Hit
ATTGAATCCTATGCATCAACTTTTAAGACATTTTCAGAAGGATGCGAAAG25510.11466652762245182No Hit
CAATAATTGTGGCCATGGTATTTTCACAAGAGGATTGTATGATAAAAGCA25340.11390238376922496No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGAT25140.11300339100072279No Hit
GTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATT24760.11129530474056866No Hit
GTTTTATTATCTGTGCGGTATCAAATGTCCCAAGCACATCCCTCATTTGT24670.1108907579947427No Hit
GCATTAATGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGC24580.11048621124891672No Hit
GGCTAAAGCATGGAACCTTTGGCCCTGTCCATTTTAGAAACCAAGTCAAA24580.11048621124891672No Hit
TTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGT24540.11030641269521627No Hit
TTCTCACTCCTCTCATTGACATCTCGATGCTTGGAGTCATGTCTGGCAAT24440.10985691631096518No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA24410.10972206739568986No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA24030.10801398113553574No Hit
GTATTTTCACAAGAGGATTGTATGATAAAAGCAGTCAGAGGTGATCTGAA23950.10765438402813488No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC23610.1061260963216812No Hit
AACTTACTCATCGTCAATGATGTGGGAGATTAATGGTCCTGAATCAGTGT23430.10531700283002925No Hit
TTTCAGAAGGATGCGAAAGTGCTTTTTCAAAATTGGGGAGTTGAACCTAT23320.10482255680735303No Hit
GTCCTGGGGTTGACCAGAGGGCCCCGGGCGCTCCGTGTGTGGCTGCGATG23260.10455285897680239No Hit
CTTCTGAAAATGTCTTAAAAGTTGATGCATAGGATTCAATCGCTGATTCG23060.10365386620830022No Hit
CTCCTGGAGTATACATCTGTTCCCAGCATGTTCCTTGAGTCAAATGCAAC22840.10266497416294783No Hit
GGTCAATACCTATCAATGGATCATCAGAAACTGGGAAACTGTTAAAATTC22730.10217052814027164No Hit
GTATACATCTGTTCCCAGCATGTTCCTTGAGTCAAATGCAACACTTCAAT22480.10104678717964392No Hit
GTAGTACATTTCCTCGTTGGTCCCGGATTCTCAAAAAACGGTCAATGCTC22360.10050739151854264No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAGCG151.2300702E-4145.00403145
GATGGCG203.86626E-4108.75301145
TTTCGCG800.099.69026145
GAGTGTT259.387235E-487.00242145
GACGGAC702.8139766E-972.502014145
CCGTTCC1400.072.502014145
CAGGGCG2050.070.72894
GGGCAGG2100.069.1211
GAATGGC851.6916601E-1068.23719145
CCGTATA902.8194336E-1064.44623145
CGCCGTT350.00356668562.144585145
GCTCGGG851.29548425E-859.769341
TAGGGGC4950.058.58095
TTAGGGG5050.057.4234624
TCTGACC2150.057.32717145
AGGGCGG2600.055.764515
GCAGGGC2800.051.783663
GCCGACC2550.051.17789145
AACTCCG2550.051.172146
CGGGGTT2700.051.0142029
Produced by FastQC (version 0.11.5)