FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_Chang_02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_Chang_02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4736472
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT143630.3032425822426481No Hit
GTTCATAAAGTTGTTGCCTTTTTGTTTCTCACCACCAAAATTAAGGAGCA115330.24349346940085362No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG97820.20652502537753836No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT92820.1959686450167973No Hit
GTACAGTGGATTTGCAAGAGCAGTGCTCAAACAAATGAGAGACCAGGAGG88540.18693238342800295No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG80430.16980993448288095No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA79800.16847983055742755No Hit
CCCCATAGACTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGT77510.16364500835220813No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA72660.1534053194022893No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT70030.1478526633325395No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC69430.14658589768925057No Hit
GCATTACCCATTGATCTATTCCTTTCTTCATCTTCAATTTTCCCTTTTAA67890.14333453253814232No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT66750.14092767781589335No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC66530.14046319708002075No Hit
GAGCCATACTGTATGAAAAGTTCCTCAAATAGCAACTGTACGAAATACAA64840.13689514051809024No Hit
GGTCAGTACAGTGGATTTGCAAGAGCAGTGCTCAAACAAATGAGAGACCA64380.13592395352490208No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG64120.13537502174614355No Hit
GAAGCACAGCTGATGATAACATATGATACACCTAAAATGTGGGAAATGGG62810.13260925009162938No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA62780.13254591180946493No Hit
TGCTAATACTGCATTACCCATTGATCTATTCCTTTCTTCATCTTCAATTT61980.13085689095174638No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACTGAT61300.12942122322268557No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA60770.12830224690444703No Hit
AGTAAGATGTTTGCCTTTTCCCCCGGTTTCAGCTTTTCAAGTTCTTCAAT60050.1267821281325003No Hit
CCTAAAATGTGGGAAATGGGAACAACCAAAGAACTGGTGCAAAACACTTA57540.1214828251914083No Hit
TCATTGGACAAAGCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTT57400.12118724654130754No Hit
GCTGTAGAGATAGGGAATGGATGCTTTGAAACCAAACACAAGTGCAACCA57340.12106056997697864No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTGTCCATTG56100.11844258764751485No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT55770.11774586654370596No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA55190.11652132642185999No Hit
GATCTATTCCTTTCTTCATCTTCAATTTTCCCTTTTAATGACATCATTCT55070.1162679732932022No Hit
TAGTTAGGACTTCTGTTTGTGGATTATAGGAAAATAGAGGGGACCCTTTC55000.11612018396815182No Hit
AGCACAGCTGATGATAACATATGATACACCTAAAATGTGGGAAATGGGAA53950.1139033440923962No Hit
ATTTATATTAGCTCAAGGCCCACCCCATAGACTCAACTGTCATTCTTTGT53610.1131855102278658No Hit
GAACCAAGCTGAGGCATCTAATTTCAGTCAAATTGGGCAAAATCCCAACA52950.11179206802024798No Hit
AAGCACAGCTGATGATAACATATGATACACCTAAAATGTGGGAAATGGGA52820.11151760213086871No Hit
TTTGAGAGCATAATTCCTCAGAAGATGGCTGGTCAGTACAGTGGATTTGC52720.11130647452365389No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT52680.11122202348076797No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA52240.11029306200902274No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT52060.10991303231603607No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACAAG51660.10906852188717678No Hit
TGACTACCCTGGTCAAGACCGCCTAAACAGACTAAAGAGAAAATTAGAGT51400.10851959010841825No Hit
GTCTGGGGGTGTCCAAATAAGTATCTGTGCACATCAATCTTATTTCTGCT51400.10851959010841825No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT51120.10792843280821676No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA50610.10685168201142116No Hit
GGTCAAGACCGCCTAAACAGACTAAAGAGAAAATTAGAGTCAAGAATAAA49890.10533156323947444No Hit
GTAAAAGGATGTCCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG49380.10425481244267884No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA48130.10161571735249358No Hit
ATCATGTACCATCTCTATCCCAATACAGGGGACATCGCATTTCTTATCTT47550.10039117723064762No Hit
CAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG47390.10005337305910392No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA11200.0123.0588762
AGCACGC11500.0119.93861
GTAACAA43050.0119.051454
CACGCAC11750.0118.467083
GAAACAC22900.0108.8958744
AAACACG23900.0103.7318345
CGCACTT14400.097.6614465
ACGCACT15100.093.615144
TAGTAAC57250.090.215652
CGGTGCA651.6370905E-1189.279972
TAGAAAC29150.088.093142
AACACGT5750.083.2070166
TAACAAG64400.079.8075945
AACACGA26100.077.212596
ACGTGCA12750.076.807032
ACACGTG6250.076.551277
ACACGAG26750.075.33727
GAGCAGA4200.074.322861
GAGACGC300.001936336972.49862145
CACGTGC13850.071.80821
Produced by FastQC (version 0.11.5)