FastQCFastQC Report
Thu 10 Sep 2020
HHH35BGXF_n02_Chang_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_Chang_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12827
Sequences flagged as poor quality0
Sequence length151
%GC40

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT230.17930926950962814No Hit
GTTCATAAAGTTGTTGCCTTTTTGTTTCTCACCACCAAAATTAAGGAGCA160.12473688313713262No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT160.12473688313713262No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG140.10914477274499104No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT130.10134871754892023No Hit
AAGCACAGCTGATGATAACATATGATACACCTAAAATGTGGGAAATGGGA130.10134871754892023No Hit
GCTAATACTGCATTACCCATTGATCTATTCCTTTCTTCATCTTCAATTTT130.10134871754892023No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTT100.0070098704145.08
CACGTGC100.0070098704145.01
TAACGCT100.0070098704145.07
GGATTGC100.0070098704145.08
GCGGAGC100.0070098704145.04
GTTTAGT100.0070098704145.01
AGCGGAG100.0070098704145.03
AAGCGGA100.0070098704145.02
TTAGTAT100.0070098704145.03
ACGTGCA100.0070098704145.02
Produced by FastQC (version 0.11.5)