FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_Chang_01_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_Chang_01_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3746570
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT92310.24638536047638238No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA88740.23685664487784827No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT73610.19647304067453697No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT71040.1896134330867967No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG67570.18035162828934198No Hit
CTATTAAGAGCGGTCGGATTTTTTCAATCTTCTTTCTTGTTGAATCATTG66980.1787768545629736No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA63080.1683673333208775No Hit
CTCATAGACTTTGGCACTCCTTCCGTAGAAGGCCCTCCTTTCAGTCCGTA62310.16631212015256622No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT61410.16390992294285173No Hit
ACTCAGCACCGTCTGGCCAAGACCAATCTACAGTATCACTATTCACGCCA60760.1621750027358357No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG59890.15985287876644505No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA57900.15454135382496523No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTTTGGCACTCCTTCCG55390.14784189271787262No Hit
GTATTAGGCGTCTCCATCCTGAATCTTGGACAAAAGAGATACACCAAGAC51050.1362579639510272No Hit
ATATAGCGGTATTCCCTCTCCTTCATGACTTGGGTCCTCAATGCTTAATT49570.13230768409505228No Hit
CCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGAAGCTCCGTCCCCGT48680.12993217796544573No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTGAA48310.128944608001452No Hit
CTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAG47730.12739652535519155No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC46460.12400675818148334No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT44080.1176542811157939No Hit
CCGCTATATGATGCAATCAAATGCATGAGAACATTCTTTGGATGGAAGGA43990.11741406139482247No Hit
CAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGA40460.1079921101167201No Hit
CCATTTACACAGGCACATTCAGACTCTTGTGTCCTCAATATTTTCTTCCT40170.10721806879358986No Hit
GCTATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGT38270.1021467635730815No Hit
ATATGATGCAATCAAATGCATGAGAACATTCTTTGGATGGAAGGAACCCA37600.10035846120584963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCCG600.0132.90826145
AGCGAAA2900.090.107361
GGTACTG9400.078.6557855
TACTGAT11100.066.612077
TTAGTAG1750.062.1654782
GTTTAAA9850.061.8499152
CCGACGC1102.5465852E-1159.314434145
ATTAGTA1950.055.835761
CTATTCG5850.055.761249
GTACTGA14350.055.0610586
GCTCACG805.880647E-754.371563145
AGGGGTT3400.053.299055
TAAACTA6450.050.572125
ACACCGC2350.049.35858145
GCAGGGG3700.048.9814263
TAGTAGA2250.048.3283423
GGGTGAC1056.921255E-848.3264057
TAGATAT7450.046.7065853
TTTAAAC7000.046.602333
AGGGTGA1255.2095857E-946.392736
Produced by FastQC (version 0.11.5)