FastQCFastQC Report
Thu 10 Sep 2020
HHH35BGXF_n02_Chang_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_Chang_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCTAAACAGCCCAAATAATGTTGTGAAACGTAAGACAAAATAAATGTA10.9523809523809524No Hit
AGCATGGTTTCCAACACTTTCGAAAACACTCTTTGTCCTGTTTCCAGCCT10.9523809523809524No Hit
GTGTGATAGCTTCTTGACATACTAGTTCAGCTAGACTTTTATAACTACTT10.9523809523809524No Hit
AAGACACACATGACCTCTTTCCCAATTTTGTTGGAAGTGCCACTGGAACA10.9523809523809524No Hit
GTGTATCCCATCCCTGCAGCAGGCTACTTAACACAACTTATAGAATTATT10.9523809523809524No Hit
TTTTTTTTTCTGTGGATGATTCGTAGAATACACAGGTGAAACCATGGGGT10.9523809523809524No Hit
AATTCAGGACCTGCATTTTCCCTGGAGTGTGAGCTCAATTTCACCGAAAA10.9523809523809524No Hit
GGTATATACAGTTACACCAAATAAAGTAATTACACAGTTGGGGAGAAAGT10.9523809523809524No Hit
TCATTGGAAGATTGTTCCCAATACTTTCAGGTACAATTGTGAGAAACAAA10.9523809523809524No Hit
GGTGTGGGGACAATTCCACGGTTTCCGCGCCTCAGCTAACGCATTAAGTG10.9523809523809524No Hit
CAAAAATAAGAGCCAAAACTGAAAAGGTAAAGCAGCTACACAAATAAACC10.9523809523809524No Hit
GGGCGGCCCGCCTGCTCCAAGGGCCGGTCCAATGGGGTGCGATAATGGGC10.9523809523809524No Hit
CAGCGGGAACAGTGTGGGTTGGGCAATTCTCTTGTGCTCAGTCACTATCT10.9523809523809524No Hit
CTTGCAGCCCTCCGAGGACTGCACAAGGCACCCATCCCGGGTCTCACTTT10.9523809523809524No Hit
TTTTTAGCACCCTCACATTATAATACAGTAATACCAATAAAATCTATTAA10.9523809523809524No Hit
GTCAAGCCTTGGTAAGGTTCTTCGCGTTGCATCGAATTAATCCGCATGCT10.9523809523809524No Hit
GAATTAGGCTGGACTGTGATCTCACCTCTGAAGAACTCTATATGGCAAGA10.9523809523809524No Hit
ATATTCCATTGCCCACGAACCGTATTGGTTGTTCAGAAGAATGTTGTATA10.9523809523809524No Hit
GCTCACGCCTTAAGTGCCCCGCCTGGGGAGGACGGCCGCAAGGCTAACAC10.9523809523809524No Hit
GTAGTGGGCGGTGGGGGTGGGCGGGGGGGGGGGGTGTGAGTTCGGTTTGG10.9523809523809524No Hit
GAGAAATGAGGTTTGTTATACAGTCTGAAGCAGAAGCAAAGAGAAGAAAG10.9523809523809524No Hit
CTACCAGAGACGTCGTGTTAACCAGGGAGGTACGACGGGCACAGTCAAAT10.9523809523809524No Hit
ACTAGGAGGAAGGTGAGGACGACCTCAAATAATCATGCCACTTATGTCTT10.9523809523809524No Hit
CGTTTGTTCAGAGGTGCTATGTTACAAAGTTTTCGAGTGAAGCACTGCTT10.9523809523809524No Hit
TGATAAGGCCTGTGGTCGGTCGCTATGCTAGCGTCTGAGAATCATGCACA10.9523809523809524No Hit
CATGTAAACATAATAAATTTACAGGAGCGCAGAGAAGCCAGTCTCTGAGT10.9523809523809524No Hit
CTTCGGGTGTTACCAACTTTCGTGACTTGACGGGCGGTGTGTACAAGGCC10.9523809523809524No Hit
GTGCTCGCGCGCAGGAGCGATACGGGTGCCCCGGTGGGGCGGGTTCACAG10.9523809523809524No Hit
GAACATGATTCATAGCCAAATCAAAATAACTAAAGCTCTCCTAATTGAAG10.9523809523809524No Hit
GACAGCCAGGAGAACCCGGAATGTCTTAAGGCCAACATACCACGCGGCTT10.9523809523809524No Hit
CCACGTTACACTAAACAGCTTCCACGTCGAAGACAACAAAAAACAAGGCG10.9523809523809524No Hit
GAGTTGGAACAGAATCTCTAGGTCCAGGTAGCACTTTCTACTCAGACAAG10.9523809523809524No Hit
GTGCAGGAGTGATGGCCCTGGTGGGTTTGGCGGGGTCACTTGGCTACCAA10.9523809523809524No Hit
TGATTAAGTGAGTAAAGAGTAGCCCCCTTGTTTTTGTTTTTGTTTTTATG10.9523809523809524No Hit
CATTTGACCGGGGGGCAAAGACTGAACTCCGCTTAATTGTTGGAGCGCTA10.9523809523809524No Hit
TAAAAACGGTTTGAAAAGAGGGCCTTCTACGGAAGGAGTGACTGAGTCTA10.9523809523809524No Hit
AACTCCGGTGTGTGGTAGCCTTGTTTGCTACACTGGAACTGATTATAGGG10.9523809523809524No Hit
GGAATTATCACTCAAGACCAGATGGTTTCAGCGGATCTCCAACTATAAGT10.9523809523809524No Hit
CTACGCGAAATATTCTTCCAAGACAGTTACTATTGTCGACACAGCTGATG10.9523809523809524No Hit
GTGTCTCTGTTTATGAATTTCCAGATTACGAAGAGTATTTGATTTGTAGT10.9523809523809524No Hit
GTCTCGGTTTGTAAGAAAAGACACTGTAAGGAGTGGGATGTAGTCAGCGT10.9523809523809524No Hit
CTACGAGGTGAGGTGGGGCGCTGGGCGTCGCGGGCCCCTCACGGCCCCTG10.9523809523809524No Hit
GCTTAATCTAATATACATAATCATCAAGGAAACAAAGACTTACAATGCCA10.9523809523809524No Hit
AGTTGGTGCAGCCTCTTTGGAGAACAATGTGGAGGTTCCTCAAAAAGCTA10.9523809523809524No Hit
GTAATCACACCGTCATCACTGAAGGTTGAGGCCGCTAACACAGATTGAAT10.9523809523809524No Hit
ATTATGGACGACAGAACAAAAATCAGGCAACTAGCCAATCTACTCACAGC10.9523809523809524No Hit
GAGGCTGTTGGGAGTGTTACTGATCCATCTATCATAGCTGCTAACTCAGG10.9523809523809524No Hit
CAAGAAGAAGCTACTCTGACAGAAGGACTTAAAGTCTAAAACATTTTACC10.9523809523809524No Hit
TGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCCACGA10.9523809523809524No Hit
GTCCCCTCCCCAAGCAGAATGAGAGACCTGTGCTGACAGATGCTGCCAGG10.9523809523809524No Hit
CTCTATTATCAACTATTCACGAAGACTGTGACAATATCAAAATAAAACAA10.9523809523809524No Hit
GCATTTAGCTTGGCCCCTGACCGCGGCTGCGGGGGAATACAGGGAACCAG10.9523809523809524No Hit
ATGATATTGGTGCTGATTGCTTCTCTTAGGTTTGTTCATATATGCTCTAC10.9523809523809524No Hit
CAGGACACAAGGGGATAAGCGTGCTGTGAGAGCCACTGGGCGGCAGAGAC10.9523809523809524No Hit
TGTCAGTTAAATATAATTTAGAATTTGCTGGATGAACCTAAACAGGACGA10.9523809523809524No Hit
ATTTAAGGTTGGTCACATCCAAATTGATGACGGATAATATTAGGGAAGCG10.9523809523809524No Hit
CATAATATATCTCACTAAGATAAAAACCAACCGACACCTCATAACAGATG10.9523809523809524No Hit
GGCCATGGGTAGTGTATTAGGAAGACTTTGCTCGAAACTTGATCAACTCG10.9523809523809524No Hit
CTCCCTGATGTATGCTGCTTCAGTGGGGCATCTCTTTGAATGGGAAGCGA10.9523809523809524No Hit
GTTGGGACATCATTGAGACAATAATACTCAGCGCATTTGATGGCTCCTAG10.9523809523809524No Hit
AATCGAGAGCTTAGGATACCGAGATGTGTGGGTACTTAAGCGATGTCTCA10.9523809523809524No Hit
GAAGCAGAAGATAAGTGAGAAGAAACGAATCTAGAACCCTCCCTTACTGG10.9523809523809524No Hit
GAAGTACATTTGTTGAATATTAAATGGTAAACAACCCTAACATTCCTGAA10.9523809523809524No Hit
ACATTCTACTGAACACCCAATTAGGTTCATCGGCAAACAAAAATAAGAAA10.9523809523809524No Hit
GTTATACACTTTATGCGCAGGGTCATTATTTGTAACGCATCACATGCGTA10.9523809523809524No Hit
GGGAAAAAGACTGGTACGGCAGAAACTTCTTCGACCTGCATCAGTTTGAT10.9523809523809524No Hit
CTACTAAAGCCCTGGCAGACAATTTGATAAGATAGAGTAAGGAGCCAGGC10.9523809523809524No Hit
CGCGTAAAGAGCGGCTGCTCTCCGCACATGAGATGGCAGAAGAACGCCTT10.9523809523809524No Hit
ATTAACAGGCTCGCTCATATCAATTTACCTCCATACACAACTGTATTTAT10.9523809523809524No Hit
CCAGAAACAGAAATCAAATTGCCTATACTCTGTAGAGAAACCAGTCACAG10.9523809523809524No Hit
GATTCTAGGTCTATTTGGTGAGTTGGTTTCAAGGTGGTTATTTTGGTTTG10.9523809523809524No Hit
CTCGAAGAGGTGCCCTCGGCCCTGCCCCGGCACCTGGAGCAGTTGAGGCT10.9523809523809524No Hit
ACGGAATGGTGGGGACTCATGGGAGACTGCCGGGGTCAACTCGGAGGAAG10.9523809523809524No Hit
GTACTCCTATCCCCTGAAGAAGTTAGTGAAACACAGGGAATGGAAAAGTT10.9523809523809524No Hit
GACCCCTTTAACACTCTGGACACTGTTCAATCATACAAACACCACAGTTC10.9523809523809524No Hit
CCCTTACTCAGATGAATGGCCAGCAGAAAAGGGAAATCCTGATTTGCAAA10.9523809523809524No Hit
CATTGCGCCGTGCGCGTCAAACAGAACTGGAGTACTGTCCCATCAGATCT10.9523809523809524No Hit
TACTTGGGATGTCTCAATAAGGGCCATGCTGGCATCCCAAAATGAGGTGG10.9523809523809524No Hit
GGGTGATCTGTGGTACCATTAGCGTGCTTTCTGTATGGTATATGCTGGTG10.9523809523809524No Hit
ACATCATACTGCCGCATATAGTTAGGACTTCTGTTTGTGGATTGTAAGAG10.9523809523809524No Hit
CCTGCCAGCCAGGTCTGCTCTGTCTGCTTCTCACGATGCCCCTGCAGCGG10.9523809523809524No Hit
GGAATACTAGGGGTGCTGGAACAATGAGAACAGAGAAGGAAGTGTAAGAA10.9523809523809524No Hit
CTGGTTAGCGAGCACGAACATATGCGTCAGACATCAGGTGAGAATAACGT10.9523809523809524No Hit
GGACAGTTTTCTGGTGGGTGACGGTAAGCCTAAGCGTGGTTGGTTGAGTT10.9523809523809524No Hit
GAGATGGGACACGATAAAGACTCAAACACTAAAACAATATCCACACACTG10.9523809523809524No Hit
CAGCAGAAGCTGGTCTGGGGCGTATTTTGGGAGCCAGCAGACAGGAGGCA10.9523809523809524No Hit
AACTAGGCTACGTAGCCAATATCTATTGTAAAATCTACCCAGTTCTGTAC10.9523809523809524No Hit
ATATTTCTATCGCTCCCAAACAAAGTTCATACCCACTAAGAAGTCACTCC10.9523809523809524No Hit
ACTCATGGGAGCTCTGGCCAGTGTTGGGCAGAGTGCCTGTGTCCTTTGCT10.9523809523809524No Hit
AACCAACAGGGCCCTTGAAGTGCTGTGGCTTCAATGTACCAGGAACTGTC10.9523809523809524No Hit
ATGGAAGAACCACCAAGTTTTGCAGAGGATGTGGAAAAAAATAAAAGGAA10.9523809523809524No Hit
GCTCCGGGGCAGGCCCAAAATCAAGCAGCCTCACCAGTAGTACACAGACT10.9523809523809524No Hit
GTTGTAAAGTGAGCAGCTCCCTACCCACAGAACTCCAGGACCAACCAAAT10.9523809523809524No Hit
TGGTTAATCTGACGACTTTGTGTAGGTTAATTTTAGTTTCCACAGAATAA10.9523809523809524No Hit
CACTATAACTTATGCTTGGATGATTTTCTACAACATGTCTTTTCTTTGTA10.9523809523809524No Hit
GAGCCTGAACTTTGCGTTGTGATCTTCTGTCTGTATTGTGGCTCATTGAC10.9523809523809524No Hit
GATATTAAATCTCTGTTAGTATAAATCCAAGGCACAGGACAAATGTGAAG10.9523809523809524No Hit
TCTGATGCTGGACTAGATCCCTGGACTCAGGCATCATGACCTGAGATGAA10.9523809523809524No Hit
TGATAATGGTATTAATGCAGTAAAATGATGCAACATTTATAAATATTTAC10.9523809523809524No Hit
CTCTAGAGCCAGAAAAGAATAATTCGCAGCTGACACTTCTGACAGCAGTC10.9523809523809524No Hit
CTAAATAGCATTGGTGTGGTTTGCTAACATTTTCTCAATAAGCCATGTTA10.9523809523809524No Hit
CTCAAAGAGTTTGATGCGGAAACTGAGAATATAGGAAGATCAAGGCGAGT10.9523809523809524No Hit
AATAATTACACTGCCGTTGCGCTCTTCCATTTCTTTGGTTCACTCCTTAC10.9523809523809524No Hit
CAGGAAAAATGTGCTTCACTGAGAAGTCAATAATTTGAGGGGGGATGACA10.9523809523809524No Hit
GAGTTTGACTTCTTCATTGCCAATTTGGATACCTTTTATTTCTCTTTGTT10.9523809523809524No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)