FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1805_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1805_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4348034
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC52670.12113520731438622No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC45950.10567994638496388No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG15250.036.145412
AGCGAAA16550.032.8978581
CGAAAGC19450.028.325563
GTGATCG4850.026.9032869
TATTGCG1701.0331771E-425.5842027
GTCCTAT24150.025.2502981
TATACTG23850.024.0100615
TATAATA29950.022.763572
GCACCGT15050.019.7472636
TCTATAC50600.019.6270313
ATAAGGT36450.019.4900573
TAATACA58450.019.34664
GTATTAT34800.018.7744661
GTAGTGC28550.018.5347338
GTTCTAT52000.018.2882561
GGTAGTG30300.018.1819537
TATTATC29650.018.1015762
ATCTAAA62250.017.9591461
GTATAAG15150.017.7293761
ATAATAC46450.017.3229473
Produced by FastQC (version 0.11.5)