FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1805_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1805_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3408846
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG1105.2731066E-639.539924
AGCGAAA15150.037.8594171
CGAAAGC18500.031.741133
ATAGCGA1652.3923585E-630.7514695
GCGAAAG18600.030.4163722
GTAATCG1255.1606377E-428.9959439
CCGGTAA4900.022.192724145
CCGTATA1650.002602917621.968555145
CTATGCG8100.020.5835384
GTACCTA10650.020.418786
ATTATAC13550.020.3307513
GCACCGT20350.019.9471916
GTATTAT21300.019.4291921
TATGCGA2256.881517E-419.3294945
GTACACC14200.018.8895873
GTGTACA18050.018.5029131
GTATTAG12200.018.4485111
CGGTACG3151.6881386E-518.4093117
CCATCGA6503.6379788E-1217.8436579
TCTATAC28900.017.8102323
Produced by FastQC (version 0.11.5)