FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1805_d10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1805_d10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTCTAAGTAGGCAGGCACTTCTGCCTGTGATGACGGATTAGACTTGAG17.6923076923076925No Hit
TTTGGTGGTTGCGCAATCATTTTCATAATTGGGCAGTATCTCCTAGTTTA17.6923076923076925No Hit
TAGAACAAATATTGTTTTAAAAAAAATAGCATAAAAAAAATATTTATTTT17.6923076923076925No Hit
TTTCTCCAGAAAATAGACCAATGCACAGCCCACTGCTCCAGTTTCCAAGG17.6923076923076925No Hit
GAGAGAGACAGCCTGAGCAAAGTGCAAGCAGCAGCAGGCAGTAGAAGAGA17.6923076923076925No Hit
CGAAAGCAGGGAAGGAGGAAAGTCAGAATTAGAAAAAAGAAAGAGACAAA17.6923076923076925No Hit
GCGTTTGAAGGAGGCTCTGGTCTTGCTAAGCTCTGTAATCTTGGTCAAGA17.6923076923076925No Hit
GTACTGTGTTCACATTCGGTACCCCGAAGAACCCCCAAGAACCACGCGGT17.6923076923076925No Hit
TAGGGAGAAGATGACTCCCCACTGAGCAGGGAGCCTGACCTGGGGTTAAA17.6923076923076925No Hit
CCTCTTCCCGTATTGTAGAATTTGCTTTGCAGATATTTGGGTGAGCATTT17.6923076923076925No Hit
TTATCGAGACCTCCCAAGTATCTGTGATGAACATAAACATAAAAATCCTC17.6923076923076925No Hit
CCACGTGCGGGGAGCGAGGCGCTTCCGGGAGGAGAAGACCGTGTCCCACC17.6923076923076925No Hit
TGGGTGAGGCAGCAAAGAGGCCCTTCTTCACACCGCCCCTCCCAAAGGTG17.6923076923076925No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers