FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1805_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1805_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5646441
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA31800.026.4833451
TATACTG26100.026.3839595
GCGAAAG32700.025.7288862
CGAAAGC33450.024.9239163
GTATATA26050.020.9023691
GTCCTAT52100.020.0662751
GATGCGC8100.018.7927868
CGACGAA5458.54925E-1118.622353145
GTAATAT29500.018.4578531
TACACCG11450.017.0928765
TCTATAC48450.016.9082723
TAGTACT16200.016.5561354
GCACCGT32450.016.5300396
GTATTAT44300.016.2245791
CCTACGG7703.6379788E-1216.005633
TATGATG62250.015.8363985
AGCACCG33500.015.7955565
TAATACG7853.6379788E-1215.6982594
TACTGTG54950.015.56592757
GTATAAC16750.015.1703381
Produced by FastQC (version 0.11.5)