FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1805_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1805_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5426493
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATATGGATGCGCTGTTTATCGATCCTGATGTGTGCGGCTGCGGCGTGG91780.16913317680498252No Hit
TCCATATGCTGCCCACTTAACAACATAAACCCAACCGGCTGATCCCGTTC83880.15457497134889883No Hit
ATCCTGATGTGTGCGGCTGCGGCGTGGGTCGGATGCTGGTGGAGCATGCG83490.15385627513018077No Hit
GGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACG68020.12534799178769787No Hit
CTATAAGAAGGTGGGTTTTAAGGTTACGGGACGCTCTGAGGTGGACGATT63410.116852633920287No Hit
GGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGAT60930.11228246309356706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCG24250.043.941759
GTCATAG27700.039.2749332
CGGTAGA6450.037.13631
CATAGTG31550.035.1527984
AGTGATC38100.030.4412737
TAGTGAT41250.028.9950456
CGAAAGC28550.028.1856923
GTGATCC49000.027.6633878
TACGGGC8200.027.40663145
ATAGTGA44800.026.69725
TATGCTG66900.026.2209875
CCATATG69550.025.548942
TATACTG24100.025.265165
GATGCGC56700.024.9293738
GATCGAT31800.024.8466117
GTTAAGG26450.024.404412
AGGATCG35200.023.8877415
GTCCTAT35400.022.5545351
CATATGC83800.022.2331473
TCCATAT76300.022.1654111
Produced by FastQC (version 0.11.5)