FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1805_d06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1805_d06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACAGAGGTGGAGAGACAATAAGTCTTAGCAAATAACTAGGGAAAACAT12.1739130434782608No Hit
ACAAAGCATACAGCTCCGCAGGGACCCAGATGAGCCCCTCAGTCCCAGGA12.1739130434782608No Hit
ACTAAGGCATGTATCACTATATACTTCCTTCTTAGCACAACTTTTTTTTT12.1739130434782608No Hit
GCCTGATCCAGGACTCCATCCAAGACTCCAGGATCATCACCAGAGCAGAA12.1739130434782608No Hit
GGTTGGAACAGCAGGACGAGAAATCAAGGCAGGCGACTTGAAAGGATAAA12.1739130434782608No Hit
GTGTTTACACTCAGACCCCTGCCTCACATCAGGCTCTGGGCGAGGCGCCT12.1739130434782608No Hit
GCACCTTAGCTCATATGTAAGGCCACAAGACAATTCATTAAATTCCAGAA12.1739130434782608No Hit
ATTTTAGCGGAATCAGGGGTCAGGAAATTAGATAGATTTGATGAAATTAA12.1739130434782608No Hit
ATTTAGTGCCACACCATCTGTAGTTTTGTTTTCTGTTGGTGATTTCATGG12.1739130434782608No Hit
GTTCTCTTCCTTCGATGACGTTATTCCTGCTGTATAGCCTTTCTAATAAA12.1739130434782608No Hit
CTGTAGTTGTAGGCACAATTGAACAGAGATGGCTCTACTGCCAATTCTTC12.1739130434782608No Hit
TATCAAATCCACACCTCAGAGCATCATGGCTTGGCCATGTGGCACTGTCA12.1739130434782608No Hit
TTATTGCAGTGTGAAGACAAAGAAATAATGAAAGACAACTTCTGCTATAA12.1739130434782608No Hit
CTATAAGGATTCTCAAGAATGTTAGTCTGTTGACACTAGAGAGTCTTTAA12.1739130434782608No Hit
GGGAAATTGAACATTCCACCACAGACTATGAACACCACACAAACCAAGTT12.1739130434782608No Hit
CCAGTAGATAATTTTATTGAGTATCTGCCACTGTGATGAATGAAATGGTG12.1739130434782608No Hit
GTATTTCTGTGTTCCTATTATGGTTTGCCCATCATCCCATCTCCTTGTTC12.1739130434782608No Hit
CAACAACCATTAGCAGAAGCCCCACCGGAACAGAGTAGGATCAAATTTTC12.1739130434782608No Hit
GAGTTTAAAGTCACACGCTATCACGCACAGATGCGTAAAGTCCACCTGAT12.1739130434782608No Hit
CTATTTGTGAAACTCCTTTATAGTCATCAATTGCAGTAGGAGATGAACAT12.1739130434782608No Hit
GCCCTAAAAAAGCAAAAATCGCTCTGGTTAGAAAAAAAGATGAAATAAAT12.1739130434782608No Hit
TCATTAAGGAGGCCTATTAGTAACTACTCTAGGTAGACATTATCAAATAT12.1739130434782608No Hit
CTAATTTTACTTTCATTGAATTAATTTAAAACATACCTCAAACTAATTCT12.1739130434782608No Hit
GTTTTAAAGGTAAAATCCTGAACCAGAAAAATTAACCCTTCTGCAGAGAA12.1739130434782608No Hit
GAAATATACTCTGCTTATTCAGAGAAACCAGCACGGCCTGTTAATTCTTT12.1739130434782608No Hit
ATTAGTGTACATAAAATATAAAGTTCTTCCTTGCCGAGTCGAATTGTCTC12.1739130434782608No Hit
GAATTTACACTGTTAAAGACGCACAAAACACAAATAACAGGGGAAACAGC12.1739130434782608No Hit
AAGTATCAAAAAATGTGTCTATAGAGTAAATTAACAAAGATAACTAGTTT12.1739130434782608No Hit
GTACACGCAAAGTCAACAGATAGCGTTTGCCCAACTTCAATGGGCATAAA12.1739130434782608No Hit
GCTCCAAATAGTCCTCTACTTGATCTAGCAGGCACGTTCCTCAGACCGAC12.1739130434782608No Hit
TAACCGATGATCTTCTGATTTTCAATACAATGCATGAGAAAAAGAAAAAA12.1739130434782608No Hit
AGATGAAACAATATTAAGTTCGGTGTTTTCTTCCTGGTATTAAAAAAAAA12.1739130434782608No Hit
TAAATCGACTGAAAAAACATTGACTGACAAAACACAACCAAGTTGCTTAT12.1739130434782608No Hit
GTCATTCTCCATCAGGTAAAGATAACAGAGGGTCAGCATCTAATTCGAAC12.1739130434782608No Hit
ACATACTACCTGGCCTACGACTTGCAACCCAGGAATCTTCATGACAGATC12.1739130434782608No Hit
ATCCATACGTGAGCAAATCCAGCAGGCCCTGGGAAGGACTTTACCTGGAG12.1739130434782608No Hit
GGTATGCAAATCACTCCATAAATTTGAGAAGTTCTAAGTCATTATTTAAA12.1739130434782608No Hit
CGATAAGGGCCGTGCTGGACTCATAAAAAGATGTGGGGAGCGAATCTTCC12.1739130434782608No Hit
ATTATCAGGCTCCTGCTACAAAAGACACACTGTCTCAGTAAGAGATAAAA12.1739130434782608No Hit
CCTTTTAACACAGATGCAAGTCTTTCCACTCCCGACGAATTCAAAACCAC12.1739130434782608No Hit
ACTGTGGTATGCCCCTTCAAATTTGGTAGAGGGACTATAGTAAGTTAGCT12.1739130434782608No Hit
GACCAATGATAAACTCATAAAAACAGGTTCCGACCAAATCTTATAAATCT12.1739130434782608No Hit
ACTTAACCCCATTGTTCTACAAAAAATATAGAAACACTCCCAAGAGCTCA12.1739130434782608No Hit
CACTCATTCTAGACAAATGCACTAATGAAGGACTGCTCTAAAGCAACACT12.1739130434782608No Hit
GAGACCACTATACCGGGCTTGATCCCAGTCCACTGGTATAATTCCATGAG12.1739130434782608No Hit
TTCTAACATGGTACCTAAAGCAATCTTTTTAAAAATTTATTGCCCTCAAT12.1739130434782608No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)