FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1805_d04_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1805_d04_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5052662
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC65930.1304856727008456No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT58380.11554305433452702No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT7100.032.7166181
AGCGAAA20100.031.0584051
CGTACGG950.00473269430.5215635
CGAAAGC21800.029.2646833
GCGAAAG21450.029.0777472
GTTGACA38550.025.9855251
TAGTCGG6400.024.9179958
GCACCGT46950.023.931316
AGCACCG46700.023.7489785
TATACCG1902.1971946E-422.891175
ACATGGT44400.021.8772225
ACCGTGT49550.021.7978178
ATAGCAC59000.021.7489813
CCGTGTT51000.021.6046929
GTCTACG4057.9489837E-1021.5081461
CACCGTG54950.021.370657
TATACTG30600.020.6095355
CTACTGA54950.020.5791446
TAGCATG53850.020.4713972
ACATAGC54750.020.152841
Produced by FastQC (version 0.11.5)