FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1805_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1805_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4392090
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC56060.12763855021185813No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG12900.044.9770242
AGCGAAA14600.039.276781
CGAAAGC15300.037.903673
ACTCCGC9350.030.2354168
TCTATAC42600.028.9282423
TATACCA52250.027.052745
TAGACCG2207.5070056E-726.359085
GTTCTAT46300.024.9274431
CTATACC49500.024.1624934
TATACTG29400.023.1762665
TTCTATA54250.021.3899962
GTCCTAT37150.019.7344111
TACCACT53500.019.5108687
GTATAAG13750.019.0047171
ATACCAC60050.018.7103396
GACGAAC3151.6866688E-518.411404145
GTACCGT6800.018.1226923
GACTCCG18000.017.719367
CGATACG2050.00914028717.679874
TATATAC21750.017.6644173
Produced by FastQC (version 0.11.5)