FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1803_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1803_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4422380
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC51380.11618178446899632No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG9900.038.8076139
GCGAAAG13900.032.3466952
CGAAAGC16550.026.7188593
AGCGAAA18000.024.1966081
GTATTAT46900.022.5972581
TAGAGTG27250.020.4821685
TATAATA36000.019.5398962
TATACTA18600.019.4944112
GAGTGAT22150.018.980647
TAATACA52500.018.6399964
TATACTG30600.018.239845
TAGTACC14750.018.1838539
ATAATAC47300.017.7783
TTAGAGT28950.017.7779274
GTATTAG20050.017.7401561
CTAGTAC12850.017.488183
GCCGACC5953.0013325E-1017.057554145
GAATAAC35550.016.9478341
AGTGATC29100.016.4405888
TAGTACT20300.016.426054
Produced by FastQC (version 0.11.5)