FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1803_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1803_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3262846
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG15500.027.1368452
TATACTG23800.024.3644395
CGAAAGC18500.023.1201293
AGCGAAA18250.023.0700191
TACACCG6250.020.8754525
GTAATAT15350.020.8078441
GTCCTAT27500.020.0615121
CCTACGG4102.1727828E-819.4499633
ACTACGC2751.2313957E-418.477711
GTATTAT27900.017.9526141
CAGTACG3652.9932307E-617.8741879
TCCTATG31950.017.4776462
CCTCGCG3453.6873214E-516.8092219
CTGATCG10450.016.6483659
AGTAGCG3554.7131616E-516.3344695
GTATATA20450.016.3285391
GTATAAG11950.015.7938581
GTCTATA12050.015.6476592
GTATAAC11900.015.250211
GTATATG23450.014.8587121
Produced by FastQC (version 0.11.5)