FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1803_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1803_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4282230
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG27650.032.772065
GTTACGT5350.032.560741
AGCGAAA19900.025.8965631
GTAATAT21600.024.5304761
GCGAAAG21300.024.5137162
ACTACGC3152.8359864E-823.0423261
TTACGTG7600.022.900972
CGAAAGC24050.022.0058043
GTATTAT47750.020.2169251
ATACTGT36550.019.4369266
AGGTCGG17850.019.0874778
CCGACTA8800.018.1233734
GTATTAG13650.018.0793651
GTGATCG7000.017.605159
GTAGCAT46600.017.2891621
CAGGTCG20650.016.4993447
GTTGACA31850.016.4081611
TAGTCGG6306.7666406E-1016.1092878
CTACTGA41050.015.5402636
GTATATA21100.015.47985651
Produced by FastQC (version 0.11.5)