FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1803_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1803_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5192911
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG22150.032.0876162
ACTACGC6950.031.3375661
TATACTG40700.029.921445
AGCGAAA24400.028.5634231
CGAAAGC28100.025.024323
TACGCAC8200.024.7537543
CTACGCA8300.024.4661182
GTATAGG17850.021.5559161
TTATACT34800.021.2467614
ATACCGA7750.020.577316
TATACCG8750.019.8824925
GTAATAT28250.019.0170041
TATAATA44450.018.9265462
TATATAC22350.017.8394763
ATAATAC44900.017.7600123
CCTTATA31500.017.7282282
GTATTAT45100.017.2240811
GTATATA19550.017.0820471
TGATCCG4701.0381336E-716.9656629
TAATACA59150.016.421844
Produced by FastQC (version 0.11.5)