FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1803_d04_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1803_d04_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4591980
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC49820.10849350389156746No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG14950.032.9901852
AGCGAAA16400.029.6576141
TATACTG34850.025.5831785
CGAAAGC20600.024.9859163
CCGACGC6400.024.920202145
GTCGGTA5200.023.700333145
TATACCG7050.022.619645
TCTATAC50950.021.9119323
GTTCTAT51650.019.9582841
TATAGCG4752.728484E-1019.8502862
TACCGAG7000.019.675117
GTATAGG17200.018.9928131
GTCCTAT46150.018.876211
TATACGG2701.08188295E-418.7925825
ATAGCGG5107.0940587E-1018.478953
CTATACC64950.018.1915634
TGCACCG4806.699338E-918.1214165
TATACCA61200.018.0029755
GTACTAG11850.017.7658021
GCCGACC8250.017.57458145
Produced by FastQC (version 0.11.5)