FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1803_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1803_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9299851
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC702190.7550551078721583No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG529480.5693424550565379No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA427020.4591686468955255No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA270660.29103692091410927No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG264500.2844131588774917No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT257840.2772517538184214No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG253140.27219790940736577No Hit
GTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGTC228690.2459071656094275No Hit
CCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTGTAAGTCACAT228490.24569210840044645No Hit
GGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCCTGTAGAATGA219850.23640163697246333No Hit
ATTCTAGACACATGCACTATTGAAGGACTAGTCTATGGCAACCCTTCTTG215920.2321757628159849No Hit
GTCTTGAACAGGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCC210660.2265197582197822No Hit
GTACTGTTTAAGCCACCTTTTTCAGTCTGACATTGCACTACACAATTACC206580.22213259115656797No Hit
CTCCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTGTAAGTCAC202480.21772391837245564No Hit
ATGTAGGACCATTCTCTTCCTCCCAACAGCAGGTCACAAGAAGGGTTGCC197660.2125410396360114No Hit
GTGTTACCCTGGGAATGTAGAAAACCTAGAGGAACTCAGGACACTTTTTA196740.21155177647469836No Hit
TGTCCATACCATGGAGCAATTAGATTCCCATTGGATCGTACTCGCAATGT195250.20994960026778925No Hit
TGTATGCCCCACACGAAAAGAATGCTCTTTCCCCTGTTATTTGTGTATTG191650.2060785705061296No Hit
GCTCCACACAGAGCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATC186080.20008922723600628No Hit
CTATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGA180560.19415364826812817No Hit
TTCTACAGGAGTATGAGATGGCTGACTCAAAAGAGCGGTTTTTACCCTGT176360.18963744687952527No Hit
GTTTAAGCCACCTTTTTCAGTCTGACATTGCACTACACAATTACCACCTT175760.18899227525258203No Hit
TCCATGCACTGATCATCACATTTATGGTATAGCTCGAAACAGCCTTTCCC171370.18427176951544708No Hit
AGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGT166530.1790673850581047No Hit
TTCTAGACACATGCACTATTGAAGGACTAGTCTATGGCAACCCTTCTTGT163960.17630389992269768No Hit
GTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGCATTCTTTTCGT158800.17075542393098556No Hit
GTATGGACACGTTCTTTCAGGAGGGAGCCATGGAAGAATCCTGAAGACTG156830.16863711042252183No Hit
GTTTTTACCCTGTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGC156690.16848657037623505No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG146390.15741112411370892No Hit
CTACAGGAGTATGAGATGGCTGACTCAAAAGAGCGGTTTTTACCCTGTTC142350.1530669684922909No Hit
ACACAATTACCACCTTTTAAATCAGTCTTCAGGATTCTTCCATGGCTCCC141090.15171210807571003No Hit
ATCTCATACTCCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTG138950.14941099593961238No Hit
CTCATACTCCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTGTA136300.1465614879206129No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC129460.13920653137345962No Hit
ATGTATGCCCCACACGAAAAGAATGCTCTTTCCCCTGTTATTTGTGTATT126640.13617422472682628No Hit
CTATATAACAAGGTGAAGAGGGCACTGGGCTCCAATGCTATGGAAGATGG124130.1334752567541136No Hit
GCACTACACAATTACCACCTTTTAAATCAGTCTTCAGGATTCTTCCATGG119920.12894830250506165No Hit
ACCTAGAGGAACTCAGGACACTTTTTAGTTCCGCTAGTTCCTACCAAAGA114680.12331380362975708No Hit
GCAATGTACTGTTTAAGCCACCTTTTTCAGTCTGACATTGCACTACACAA114620.12324928646706276No Hit
TGCTAGTACTACTTGAAAATCAAAAAACACTCGATGAGCATGATGCGAAC114440.12305573497897977No Hit
GAGCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATCCCCTCATTCT113830.12239981049158744No Hit
ACATTGCACTACACAATTACCACCTTTTAAATCAGTCTTCAGGATTCTTC111440.11982987684426341No Hit
GGTTTGAAGGTCCTATTCAAATCTTCTGTTGTCACGCTTGTTGTTGTGTC109550.11779758621939213No Hit
TTCCAGTGTAAGTCACATTCCAGGTTGTGTCTGGGAAGATTTGGATTCTT108230.11637820864011691No Hit
GCTGTAAATGGAACGTGTTACCCTGGGAATGTAGAAAACCTAGAGGAACT106460.11447495234063428No Hit
GTTATTTGTGTATTGGGCGTCTTGAACAGGGTAAAAACCGCTCTTTTGAG103120.11088349695065006No Hit
CTACTAATAGAGGGCACAGCCTCATTGAGTCCAGGGATGATGATGGGCAT102970.11072220404391424No Hit
ATGGAATGCTGTGTGCAACAAGCCTGGGACATCCCCTCATTCTAGACACA102760.1104963939744841No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG102500.1102168196028087No Hit
GCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATCCCCTCATTCTAG102470.11018456102146153No Hit
GCTTGTTCCAGTGTAAGTCACATTCCAGGTTGTGTCTGGGAAGATTTGGA99320.10679740998000935No Hit
GCCTGGGACATCCCCTCATTCTAGACACATGCACTATTGAAGGACTAGTC97900.10527050379624363No Hit
CGCTAACAGAAACCAATGTTCCTGTGACACATGCCAAAGAATTGCTCCAC97350.10467909647154561No Hit
GGGTAACACGTTCCATTTACAGCTGATGATCTTTCGACGATGTAGGACCA97260.10458232072750413No Hit
AGCCTGGGACATCCCCTCATTCTAGACACATGCACTATTGAAGGACTAGT96900.10419521775133816No Hit
CCATTGACAAGGGGCCTTGGCCCTATCACTGGTTTGAAGGTCCTATTCAA95840.10305541454373839No Hit
TGTCAATGGTCTGCAGGGAAGAATTGATTATTATTGGTCGGTACTAAAAC95810.10302315596239123No Hit
GTCCATACCATGGAGCAATTAGATTCCCATTGGATCGTACTCGCAATGTT95310.10248551293993849No Hit
GTGTAAGTCACATTCCAGGTTGTGTCTGGGAAGATTTGGATTCTTTGGTA94980.1021306685451197No Hit
GAACATGCTCTGCTTGTTCCAGTGTAAGTCACATTCCAGGTTGTGTCTGG94060.10114140538380668No Hit
ATATTGTGGAATGGCAATGTACTGTTTAAGCCACCTTTTTCAGTCTGACA93540.10058225664045584No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGTA29200.087.431872
GGTACTG33450.076.060935
CAGGTAC36450.069.807153
GTACTGA41350.068.716536
TATACTA31050.067.5065842
AGGTACT44150.059.4353524
CGCAATT5200.057.15842145
CCGTATA9350.055.04862145
GACAGTA41800.052.7175187
GACTTAA10100.049.525333145
GGATAGC83300.049.512855
TAACTAT16850.049.107381
TTACGCG450.00964786348.3254284
TATGGAT91900.046.0111352
AACTATA18700.044.991072
TTTGGAC49150.044.984893
ATACTAC48250.044.0210763
ACTATAC19800.043.568453
TTGGACA66600.042.665694
GTATAGG278000.040.652351
Produced by FastQC (version 0.11.5)