FastQCFastQC Report
Thu 10 Sep 2020
HHH35BGXF_n02_1803_d02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1803_d02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTATCACCAACCTTTTCAAGTTAAATATACTCAGCTCCGTACAATTTA11.5873015873015872No Hit
TGTTTACCTTGTCGTGGAAATGGAAGGTGTGTGAGGAAGGGGACAGGGTG11.5873015873015872No Hit
GAATTTAACATATATGTAGTCGATATTCTCAGAGATTGATGAGTCTACCA11.5873015873015872No Hit
TAGTGATGTATTGTAGAGACAGAGAGTGCACCAGCATATGCTAGAGCAGG11.5873015873015872No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGAACTGCTGTTGGGAGG11.5873015873015872No Hit
GTGCAAGATGGCACATTTCTTAGTGTAGGGCTTGGCTACTTTAATATTTA11.5873015873015872No Hit
CTTCGGAGGTGACCCTGGCTGACCCTGAACCGTTGGAACTGTCTGGGTGT11.5873015873015872No Hit
AACCAGGACCAGAACCAGGGCTGGAAACAGGACTGGAGCCAGGACTGGCA11.5873015873015872No Hit
CTGCTAGGGAAGAATTTCTCGAATATCCTGTTCCTGTTAAATGGCTATAT11.5873015873015872No Hit
CATCGCAAGTTGGCCAGTACTGGGTTTCTTCAAGATCTGTAATTGGAAAA11.5873015873015872No Hit
GAATGAACCTGAACATGCTCTGCTTGTTCCAGTGTAAGTCACATTCCAGG11.5873015873015872No Hit
GTTGTAATGTGTTCTCTCTTCAATATATTGCTGAATTGAACTTGCTAATT11.5873015873015872No Hit
GCATGAGTCATCATGGACACGCGTCCTTAGTTGCCCAGCTTATGAATTAT11.5873015873015872No Hit
TATTTATATCGAGAGAAGAGCATCTTGGGGGACTGGAGATGCATTAAATC11.5873015873015872No Hit
TTCCAATCACATTGACGGTTCGGTGCCCTCTAAGCCCAGCATGGGTGGGA11.5873015873015872No Hit
CCCTCACAGGGGGAATGATGCCTCTTGCTGCTCGGGCGGGCTGCAGGGAG11.5873015873015872No Hit
ACCCTGGGATTATGCCCTGAGCTCAAGGCAGATGCTACACCGACTGAGCC11.5873015873015872No Hit
ACTGAAGGAAGTGGGGCCACAGGGACCCTCGTTCCCCCAAGTCACACCTC11.5873015873015872No Hit
GAATACAGAATTGAGAAGAAATGCACAGCAGTACCCCATTTGCAAATATT11.5873015873015872No Hit
GAATAATGATCAAGTCAAAGCAGCTAGCAACTGCTCCTCCTACACACTGG11.5873015873015872No Hit
CTGACAGCCTGTTGTAGTTCAGGCGAATGAAGGCAAGGTTGGGGAAACCC11.5873015873015872No Hit
GCATTCCTTCCTCTTGTTTTTCTGGCAGAGTTGGAGAAGGATTGGTATTC11.5873015873015872No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.5873015873015872No Hit
CTGTAAATAGTGAAAACAAGAGTGTAGAAACAAGAGGTAGCAACAAGAGT11.5873015873015872No Hit
GCATGGGGGGGGGTAGCGCAAATTTGGGGCTAAGCCAGGTGGCCTTTTGG11.5873015873015872No Hit
CTTGAGAGGAGTATATTCCATTTGATGCTGTTGGGTGGAGGGTTCTGTGT11.5873015873015872No Hit
CCCCTGTGGTTGGAAAAGATGTTTGAGTTGAGTTCCGTCCTCATTTGGCA11.5873015873015872No Hit
TTTCTAATCCTCGGCAAAGAAGACAAAAGATATGGACCAGCATTGAAGAG11.5873015873015872No Hit
CAGGGGGAGAGGTAGGCAGAGGGAGAGGGAGAAGCAGGCTCTCCACAGAG11.5873015873015872No Hit
ATGGACCAGGCAGTAATGGGTAAAACCATCATATTGAAAGCAAACTTTAG11.5873015873015872No Hit
TCTTAGTAGGTTGTATGTTTCTGGAAATGTATCCTTTTCTTTTGGATTAT11.5873015873015872No Hit
TGTCAAGAACATGGAGCAATTAGATTCCCATTGGATCGTACTAGCAATGT11.5873015873015872No Hit
GACATAATGAGGTTTCTTGCAGGTGAAGCTCTGCAAGTCACCAAAATGTC11.5873015873015872No Hit
GGACAATAGGTACTGAGCTCTTAGAGCAGACCAGGATTTCCCATGAATAT11.5873015873015872No Hit
GGCCTATTGCAGTCCGCAAGAGCATTTCCCCTACTTCAAGAACACAGTAC11.5873015873015872No Hit
ACTGTAAATTCATAAACACAGAGACTAAACAACATTCTACTGAACAACCA11.5873015873015872No Hit
ATTGAAGCCCAAGTATATTTCAAACCAGCATGTGAGCTGCAGATGCTAGG11.5873015873015872No Hit
ATTTTTCGGCTGGTGTTGCGTTCACCTCTCTCATGGTCAGTTATGGTCCA11.5873015873015872No Hit
CGAGGTGAGCTAACTGAGGGTCCTCATGATAAAGATTCAGCCAAGTGATA11.5873015873015872No Hit
AGGGAAACCAACGCACACACAACCCCTCCTGGCACTCCGCCACGCCCCGG11.5873015873015872No Hit
CCTAAGGGAGTGGAGGAAGGCTCAATCGGGAAGGTGTGCAGACCCTTACT11.5873015873015872No Hit
CGCCTTGTCTGCACAACTGCGATCCACAGATCATGCACTTGGACCACGGG11.5873015873015872No Hit
CTTCAAGATATCAGCAAATTCTTCTTTCTTAATCCTTCCAGCCTCGAAGT11.5873015873015872No Hit
TCATTATAGGCAACTAAGTCTTTGATTTGTGCATCAATCTGATTATTGAT11.5873015873015872No Hit
GTATTAAACGCAATGCCCATAATTATGATCCTAGGCCTTTCATCTGCTCC11.5873015873015872No Hit
GATGGATACTTTTCTAAATTTATGGAGTTGCTTTTTCTTTTTAAAGTTTC11.5873015873015872No Hit
CTTATAAGTAAAATTTTTGTGGTGATGGGGTGGGGGAATGCCGCCCGTCA11.5873015873015872No Hit
AAGTTTATTCCCATCCCCAGAAACTAGCACAGGGAGTGCGCAGGGAAGTG11.5873015873015872No Hit
GCTCTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTCTT11.5873015873015872No Hit
GCGTTACAACGAGCAACTCAATTATCAGAAAACCACGAGTCTCCAACGTT11.5873015873015872No Hit
CGTTAGTGCCAGTTCCGGCATGAGAGATGCAGTACTGTTGGAATCTATAT11.5873015873015872No Hit
GTGCTCACAAGCAGAAATGATGGCTAATTATGAGTGTACATCAAGTCACT11.5873015873015872No Hit
ATGCAGGGCTCGATCCCAAGACTCTGAGATCATGACCTGAGCCGAAGGCA11.5873015873015872No Hit
GTGGGAGGAGCCAGTAGATTGATGACCTCATGGTACAGGTGACACCCCTG11.5873015873015872No Hit
AATTTGAACCTGGGCCCACTGGGGTCAGAGATGCATATGGATTGACTGGG11.5873015873015872No Hit
GCTTCAATGTCTGAGCCGCTCAGTCACCTCGCTAAAGTCATTTTGTGCTT11.5873015873015872No Hit
ATTCTTTATCACTCTAACTCCAATGCTCATGTCAGCCGATTCATTAATTC11.5873015873015872No Hit
GAAATGACTCTTGAGGAAATGTCAAGGGATTGGTTAATGCTCATTCCCAA11.5873015873015872No Hit
ACAGATACACCTACCGATGCCACAGAGGTGATACACAAATTCAAACTAGA11.5873015873015872No Hit
ATTGTGGTCCAGGTTGCGCTGCATGATGTTCTTGAGGCCCTGGAAGGTGT11.5873015873015872No Hit
ATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATCATTATTA11.5873015873015872No Hit
CTGCAAGAATTACAACCGGCCAAAATAGATGACTCACTGGAACATTTCGA11.5873015873015872No Hit
GGCTTCACAGCCCCCCTTTATCTGGATTGCCAGATCAGGAAGGAGGGAAT11.5873015873015872No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGGG50.0145.03
GAAGAAT50.0145.09
CTAGGGA50.0145.04
AGGGAAG50.0145.06
TCTGACT50.0145.0145
GGAAGAA50.0145.08
TGCTAGG50.0145.02
CTGCTAG50.0145.01
GGGAAGA50.0145.07
TAGGGAA50.0145.05
Produced by FastQC (version 0.11.5)